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Jordan Prox Thesis-Final.pdf (768.17 KB)

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Signaling Mechanisms Involved in the Regulation of Histone Deacteylase Activity in Oligodendrocyte Precursor Cells

Prox, Jordan Daniel
http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1399030894

Abstract Details

2014, BS, Kent State University, College of Arts and Sciences / Department of Chemistry and Biochemistry.
Multiple Sclerosis (MS) is a neurological disease characterized by myelin destruction and loss of oligodendrocytes. Our current understanding of the mechanisms responsible for this complex disease includes contributions from both genetic and environmental factors. The influence of environmental factors can be mediated by epigenetic modifications such as the acetylation of histones. Histone deacetylases (HDACs) have been targeted as one of the enzymes regulating signal transduction in cells. We have hypothesized that epigenetic mechanisms, specifically protein lysine acetylation, may be involved in the CNS pathology of MS through modifications to proteins involved in transcriptional events important for neuron and oligodendrocyte (OL) function and survival. To our interest, we have looked into these epigenetic mechanisms and collected data from oligodendrocyte precursor cell (OPC) cultures to show that patterns of acetylation are changed in respnse to alterations in glutamate levels. Analysis of data from immunoprecipitation and western blotting suggest that changes in the levels of glutamate triggers a signaling process in which HDACs are inactivated by phosphorylation and are transported from the nucleus to the cytoplasm by a protein called 14-3-3. This suggests a connection in glutamate-induced activation of signaling intermediates with alterations in histone acetylation through regulation of HDAC activity.
Ernest Freeman, Ph.D (Advisor)
Jennifer McDonough, Ph.D (Committee Member)
Paul Sampson, Ph.D (Committee Member)
Ruth Leslie, Ph.D (Committee Member)
39 p.
Biochemistry; Neurosciences
Multiple Sclerosis; histone deacetylase; glutamate; oligodendrocytes

Recommended Citations

Citations

  • Prox, J. D. (2014). Signaling Mechanisms Involved in the Regulation of Histone Deacteylase Activity in Oligodendrocyte Precursor Cells [Undergraduate thesis, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1399030894

    APA Style (7th edition)

  • Prox, Jordan. Signaling Mechanisms Involved in the Regulation of Histone Deacteylase Activity in Oligodendrocyte Precursor Cells. 2014. Kent State University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1399030894.

    MLA Style (8th edition)

  • Prox, Jordan. "Signaling Mechanisms Involved in the Regulation of Histone Deacteylase Activity in Oligodendrocyte Precursor Cells." Undergraduate thesis, Kent State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1399030894

    Chicago Manual of Style (17th edition)

ksuhonors1399030894
591
© 2014, all rights reserved.
This open access ETD is published by Kent State University Honors College and OhioLINK.