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Full text of this paper is not available in the ETD Center. Copies may be available for inter-library loan from Kent State University or may be available for purchase from Proquest/UMI

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The Role of Huwe1 in Female Infertility

Crawford, Katherine
http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1556459729604

Abstract Details

2019, BS, Kent State University, College of Arts and Sciences / Department of Biological Sciences.
About 10%, or 6.1 million women in the United States are infertile. Infertility affects 1 in 6 couples and female infertility is responsible for one third of all cases. The aim of our study is to investigate how the loss of the Huwe1 gene affects female infertility, especially oogenesis. Huwe1 is a ubiquitin E3 ligase and tags various proteins for degradation via the ubiquitin-proteasome pathway. Utilizing the Zp3-Cre/loxP system, we generated an oocyte-specific Huwe1 knockout mouse line. When Huwe1 heterozygous females were bred with wild-type males, the number of litters was significantly reduced and, more importantly, the Huwe1 knockout allele was not passed on to the litters. Interestingly, the oocyte-specific homozygous deletion of the Huwe1 gene caused complete female infertility. The ovaries of Huwe1 knockout mice were significantly smaller. Moreover, after PMSG stimulation, a significantly smaller number of oocytes at the germinal vesicle (GV) stage were collected from ovaries of Huwe1 knockout mice compared to those of control littermates. In vitro oocyte maturation assays further revealed that almost all of the knockout oocytes died or remained arrested at the GV stage whereas the majority of Huwe1 wild-type oocytes matured to meiosis II through germinal vesicle break down (GVBD). These results suggest that the loss of Huwe1 negatively affects oogenesis, resulting in female infertility. Potential molecular mechanisms will be discussed.
Manabu Kurokawa, Dr. (Advisor)
Gail Fraizer, Dr. (Committee Member)
Alexander Seed, Dr. (Committee Member)
Scott Bunge, Dr. (Committee Member)
50 p.
Biology; Biomedical Research
Huwe1, female infertility

Recommended Citations

Citations

  • Crawford, K. (2019). The Role of Huwe1 in Female Infertility [Undergraduate thesis, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1556459729604

    APA Style (7th edition)

  • Crawford, Katherine. The Role of Huwe1 in Female Infertility. 2019. Kent State University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1556459729604.

    MLA Style (8th edition)

  • Crawford, Katherine. "The Role of Huwe1 in Female Infertility." Undergraduate thesis, Kent State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1556459729604

    Chicago Manual of Style (17th edition)

ksuhonors1556459729604