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Full text of this paper is not available in the ETD Center. Copies may be available for inter-library loan from Kent State University or may be available for purchase from Proquest/UMI

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DEVELOPMENT OF AN IN-VITRO MODEL OF MYELINATION, DEMYELINATION, AND REMYELINATION IN MATURE OLIGODENDROCYTE (MO3) CELLS

Pokhrel, Bishal
http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1621027876981607

Abstract Details

2021, BS, Kent State University, College of Arts and Sciences / Department of Chemistry and Biochemistry.
Multiple Sclerosis (MS) is a neurodegenerative disease that causes depletion of neuronal myelin sheath impairing nerve impulse conduction. The death of oligodendrocytes in the Central Nervous System (CNS) causes this disease. The current treatment methods for MS are immunoregulatory and do not promote the repair of lost myelin. Therefore, the development of a model to replenish the destroyed myelin is essential. This study is about an in vitro model to demonstrate myelination, demyelination, and remyelination of oligodendrocytes. A dose-dependence relation between a chemical differentiating agent, PMA, and oligodendrocyte differentiation is studied. Lysolecithin, a membrane integrating phospholipid, is used to mimic the in vivo condition of demyelination during the diseased state. The goal of this experiment is to promote remyelination via the use of gold nanoparticles (AuNP). N-acetyl aspartate (NAA), an essential metabolite for myelin production, is delivered using AuNP to replenish the myelin. The different levels of Myelin Oligodendrocyte Glycoprotein (MOG) mRNA expression levels are studied via qRT-PCR. Western Blots and HPTLC are used to confirm an increase in the levels of myelin proteins and myelin lipids respectively to demonstrate remyelination.
Soumitra Basu, Ph.D. (Advisor)
Sanjaya Abeysirigunawardena, Ph.D. (Committee Member)
Jennifer McDonough, Ph.D. (Committee Member)
Alexander Seed, Ph.D. (Committee Member)
Biology; Chemistry; Neurosciences

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Citations

  • Pokhrel, B. (2021). DEVELOPMENT OF AN IN-VITRO MODEL OF MYELINATION, DEMYELINATION, AND REMYELINATION IN MATURE OLIGODENDROCYTE (MO3) CELLS [Undergraduate thesis, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1621027876981607

    APA Style (7th edition)

  • Pokhrel, Bishal. DEVELOPMENT OF AN IN-VITRO MODEL OF MYELINATION, DEMYELINATION, AND REMYELINATION IN MATURE OLIGODENDROCYTE (MO3) CELLS. 2021. Kent State University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1621027876981607.

    MLA Style (8th edition)

  • Pokhrel, Bishal. "DEVELOPMENT OF AN IN-VITRO MODEL OF MYELINATION, DEMYELINATION, AND REMYELINATION IN MATURE OLIGODENDROCYTE (MO3) CELLS." Undergraduate thesis, Kent State University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1621027876981607

    Chicago Manual of Style (17th edition)

ksuhonors1621027876981607