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The Effect of Heptyl Paraben on Melanoma Cells From A Mitochondrial Perspective

Kinney, Emily Lee
http://orcid.org/0000-0003-0269-2584
http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1652112841612308

Abstract Details

2022, Bachelor of Science, Marietta College, Chemistry.
After finding that heptyl paraben, a novel paraben, causes melanoma cells to die with a LC50 value of 0.195 millimolar when treated for an hour, the next step in this project was to determine how the cells die; they may die through apoptosis or necrosis. Parabens are esters of p-hydroxybenzoic acid and are used as preservatives. Melanoma is a type of cancer that starts in skin cells. Apoptosis was proven to be induced through the caspase assay procedure. During apoptosis, caspases are activated, so by showing caspase activity is increased with increased concentration of heptyl paraben, apoptosis is proven to be induced. After this, the cytochrome c concentrations in the mitochondria and in the cytoplasm of the cell were standardized using total protein concentration and compared. There was no change in the amount of cytochrome c in the cytoplasm or in the amount of cytochrome c in the mitochondria as the concentration of heptyl paraben was increased, which indicates that intrinsic apoptosis did not occur. These results lead to the conclusion that extrinsic apoptosis was induced when the melanoma cells were treated with heptyl paraben.
David Brown (Committee Member)
Suzanne Parsons (Advisor)
35 p.
Biochemistry; Cellular Biology
parabens; melanoma; melanoma cells; extrinsic apoptosis; cytochrome c

Recommended Citations

Citations

  • Kinney, E. L. (2022). The Effect of Heptyl Paraben on Melanoma Cells From A Mitochondrial Perspective [Undergraduate thesis, Marietta College]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1652112841612308

    APA Style (7th edition)

  • Kinney, Emily. The Effect of Heptyl Paraben on Melanoma Cells From A Mitochondrial Perspective . 2022. Marietta College, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1652112841612308.

    MLA Style (8th edition)

  • Kinney, Emily. "The Effect of Heptyl Paraben on Melanoma Cells From A Mitochondrial Perspective ." Undergraduate thesis, Marietta College, 2022. http://rave.ohiolink.edu/etdc/view?acc_num=marhonors1652112841612308

    Chicago Manual of Style (17th edition)

marhonors1652112841612308
108
© 2022, all rights reserved.
This open access ETD is published by Marietta College Honors Theses and OhioLINK.