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Analysis of Methoxy-polyethylene Glycol-modified Human Serum Albumin

Houts, Frederick William
http://rave.ohiolink.edu/etdc/view?acc_num=mco1149010508

Abstract Details

2006, Master of Science in Biomedical Sciences (MSBS), University of Toledo, College of Graduate Studies.
Modification of blood solutes with methoxy-polyethylene glycol (PEGylation) increases their size and half life in vivo. With three PEG reagents, we have created several species of PEGylated human serum albumins and examined their properties in vitro. Determination of size was undertaken with gel filtration, SDS-polyacrylamide gel electrophoresis, and surface enhanced laser desorption/ionization time of flight (SELDITOF)mass spectrometry. All demonstrated a significant increase in the size of the PEGylated albumin species, which was reflected in significant nonideal behavior during colloid osmotic pressure (COP) studies. Urea-induced protein unfolding experiments demonstrated that PEGylation does not significantly alter the stability of albumin’s fluorophore, tryptophanyl residue 214 (Trp 214). Solute-quenching studies of all but the most heavily modified albumin species monstrated no significant change in the fluorescent properties Trp 214. We believe PEGylated albumins have promise as resuscitative agents for hypoperfusion secondary to shock.
John Dignam, Ph.D. (Advisor)
69 p.
Albumin; PEGylation; Colloid Osmotic Pressure; Protein Unfolding; Fluorophore Quenching; Shock

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Citations

  • Houts, F. W. (2006). Analysis of Methoxy-polyethylene Glycol-modified Human Serum Albumin [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1149010508

    APA Style (7th edition)

  • Houts, Frederick. Analysis of Methoxy-polyethylene Glycol-modified Human Serum Albumin. 2006. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1149010508.

    MLA Style (8th edition)

  • Houts, Frederick. "Analysis of Methoxy-polyethylene Glycol-modified Human Serum Albumin." Master's thesis, University of Toledo, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=mco1149010508

    Chicago Manual of Style (17th edition)

mco1149010508
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© 2006, all rights reserved.
This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.