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mco1164046331.pdf (586.44 KB)
ETD Abstract Container
Abstract Header
Effects of Bacterial Products on Human Blood Leukocytes
Author Info
Smith, Laura Ann
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=mco1164046331
Abstract Details
Year and Degree
2006, Master of Science in Biomedical Sciences (MSBS), University of Toledo, College of Graduate Studies.
Abstract
Tumor necrosis factor alpha (TNF-alpha) is a protein that can be produced in large amounts by tissue macrophages and monocytes in response to bacterial products. Systemic release of TNF-alpha has been shown to mediate severe septic shock. Fever, hypotension, cardiovascular changes, disseminated intravascular coagulation (DIC), multiple organ failure and death are characteristics of this disease state. Our study has indicated that the bacterial products, lipopolysaccharide (LPS) and formylated peptides (fMLP), each induce activation of cytokine gene transcription in leukocytes. Interestingly, fMLP induces some intracellular signaling events that differ from those elicited by LPS. Both stimuli, however, activate the signaling pathways that converge to produce TNF-alpha. We have found that the bacterial products, fMLP and LPS, can act synergistically in the induction of TNF-alpha mRNA in monocytes, but is not seen in TNF-alpha protein levels. Our results demonstrate that multiple bacterial products may regulate the production of pro-inflammatory cytokines in a synergistic manner. Control of this inflammation is best understood at the level of synergistic regulation of intracellular signaling. Our results have also exposed a novel and potentially important pathogenic mechanism occurring during bacterial infection. Thus, our overall hypotheses are that leukocyte activation is regulated by multiple bacterial products that operate synergistically by activating multiple signaling pathways,and that this synergy is likely to play a significant role in the induction of host defense to bacterial infections and in the pathogenesis of inflammatory disorders.
Committee
Z. Kevin Pan, Ph.D. (Advisor)
Pages
95 p.
Keywords
CMLP
;
TLR4
;
Sepsis
;
TNF-alpha
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Citations
Smith, L. A. (2006).
Effects of Bacterial Products on Human Blood Leukocytes
[Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1164046331
APA Style (7th edition)
Smith, Laura.
Effects of Bacterial Products on Human Blood Leukocytes.
2006. University of Toledo, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=mco1164046331.
MLA Style (8th edition)
Smith, Laura. "Effects of Bacterial Products on Human Blood Leukocytes." Master's thesis, University of Toledo, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=mco1164046331
Chicago Manual of Style (17th edition)
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Document number:
mco1164046331
Download Count:
652
Copyright Info
© 2006, all rights reserved.
This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.