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Translational Control in Escherichia coli: Hfq and PvuII

Kaw, Meenakshi Kaul
http://rave.ohiolink.edu/etdc/view?acc_num=mco1179943314

Abstract Details

2007, Doctor of Philosophy in Medical Sciences (Ph.D.), University of Toledo, College of Graduate Studies.
In bacteria which lack a nucleus, transcription and translation are concurrent and the majority of gene regulation occurs at the transcriptional level. However, regulating translation can yield very rapid responses, and there is increasing evidence of its role in bacteria. Hfq, an RNA-binding chaperone protein is a global regulator that binds the regulatory RNA DsrA and facilitates its interaction with rpoS mRNA. This interaction controls translation of rpoS, which specifies an important transcription factor. The present study explored the possibility that Hfq needs more than one binding site to make such interaction possible. Through mutational analysis of hfq, the in vivo interactions between Hfq, DsrA and rpoS mRNA were studied. The role of cis-acting translation control signals was also explored. The PvuII type II RM was studied to understand the role of translation control in a system requiring tight, time-dependent regulation. It was found that pvuIIC translation regulates the translation of pvuIIR and this control involves coupling.
Robert Blumenthal (Advisor)
192 p.
translation; Hfq; pvuII; coupling; C protein; RpoS

Recommended Citations

Citations

  • Kaw, M. K. (2007). Translational Control in Escherichia coli: Hfq and PvuII [Doctoral dissertation, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1179943314

    APA Style (7th edition)

  • Kaw, Meenakshi. Translational Control in Escherichia coli: Hfq and PvuII. 2007. University of Toledo, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1179943314.

    MLA Style (8th edition)

  • Kaw, Meenakshi. "Translational Control in Escherichia coli: Hfq and PvuII." Doctoral dissertation, University of Toledo, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=mco1179943314

    Chicago Manual of Style (17th edition)

mco1179943314
667
© 2007, all rights reserved.
This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.