Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


mco1194979870.pdf (2.29 MB)

ETD Abstract Container

Abstract Header

Base excision repair of 7, 8-dihydro-8-oxoguanine in DNA mismatch repair proficient and mismatch repair deficient human cells

Li, Tai
http://rave.ohiolink.edu/etdc/view?acc_num=mco1194979870

Abstract Details

2007, Master of Science in Biomedical Sciences (MSBS), University of Toledo, College of Graduate Studies.
Oxidative damage is one of the most common types of DNA damage and is considered a key factor in carcinogenesis. A major form of oxidative DNA lesion is 7, 8-dihydro-8-oxoguanine (8-oxoG). The plasmid p220-12-8-oxoG:A containing the 8-oxoG located on the H-Ras codon 12 was either used to directly transform DH5á and NR12397 competent cells or after the plasmid had been incubated with HeLa, DLD1, and HCT 116 nuclear extracts. The repair efficiency of different cell lines was assessed. We also demonstrated that 40 mer Ras 8-oxoG located on H-Ras codon 12 opposite to A or C incubated with HeLa, DLD1, HCT116+3, HCT116 and LoVo nuclear extracts in BER nicking assay showed cell incision ability that uncorrelated with the levels of hMYH expression in different cell lines. We propose that the 8-oxoG:A DNA lesion located on the H-Ras codon 12 is difficult to be repaired, and that the MMR protein hMutSá may interfere with the 8-oxoG:A BER repair at codon 12. And hMutLá may contribute to the MYH in BER.
Kandace Williams (Advisor)
57 p.
Biology, Molecular
H-Ras codon 12; mismatch repair deficient; 7, 8-dihydro-8-oxoguanine; mismatch repair proficient; base excision repair; human cancer cell

Recommended Citations

Citations

  • Li, T. (2007). Base excision repair of 7, 8-dihydro-8-oxoguanine in DNA mismatch repair proficient and mismatch repair deficient human cells [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1194979870

    APA Style (7th edition)

  • Li, Tai. Base excision repair of 7, 8-dihydro-8-oxoguanine in DNA mismatch repair proficient and mismatch repair deficient human cells. 2007. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1194979870.

    MLA Style (8th edition)

  • Li, Tai. "Base excision repair of 7, 8-dihydro-8-oxoguanine in DNA mismatch repair proficient and mismatch repair deficient human cells." Master's thesis, University of Toledo, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=mco1194979870

    Chicago Manual of Style (17th edition)

mco1194979870
473
© 2007, all rights reserved.
This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.