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Replication of Adeno-Associated Virus in Murine Fibroblasts with Mouse Adenovirus Provided Helper Functions

Bhrigu, Vipul
http://rave.ohiolink.edu/etdc/view?acc_num=mco1242316433

Abstract Details

2009, Doctor of Philosophy in Biomedical Sciences (Ph.D.), University of Toledo, College of Medicine.
The adeno-associated virus (AAV) replicates to high titer when host cellare coinfected with a helper virus. Here we analyzed the coinfection of AAV and mouse adenovirus (MAV-1) in murine fibroblasts. We observed that MAV-1 coinfected 3T3 cells produced approximately 10-40 fold less AAV DNAse resistant particles (DRP) than Hela cells. Levels of AAV DNA replication were approximately 30 fold less in 3T3 cells as compared to Hela cells coinfected with human adenovirus (Ad-5). A study of these lower levels of infection in 3T3 cells compared to Hela cells revealed that receptor binding and internalization of AAV in 3T3 and Hela cells was comparable. However, AAV did not enter into the nucleus of 3T3 cells as efficiently as it does in Hela cells. Furthermore, viral DNA replication levels of AAV DNA were found to be lower in 3T3 cells than Hela cells even after transfection of proviral plasmid indicating a defect in support for the AAV DNA replication as well in 3T3 cells as compared to Hela cells.
James Trempe, Ph.D. (Committee Chair)
Manohar Ratnam, Ph.D. (Committee Member)
William Gunning, Ph.D. (Committee Member)
Ivana de la Serna, Ph.D. (Committee Member)
Randall Worth, Ph.D. (Committee Member)
157 p.
Molecular Biology; Virology
Adeno-Associated Virus; Adenovirus; Murine Fibroblasts; Hela; Mouse; AAV genome copy

Recommended Citations

Citations

  • Bhrigu, V. (2009). Replication of Adeno-Associated Virus in Murine Fibroblasts with Mouse Adenovirus Provided Helper Functions [Doctoral dissertation, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1242316433

    APA Style (7th edition)

  • Bhrigu, Vipul. Replication of Adeno-Associated Virus in Murine Fibroblasts with Mouse Adenovirus Provided Helper Functions. 2009. University of Toledo, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1242316433.

    MLA Style (8th edition)

  • Bhrigu, Vipul. "Replication of Adeno-Associated Virus in Murine Fibroblasts with Mouse Adenovirus Provided Helper Functions." Doctoral dissertation, University of Toledo, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=mco1242316433

    Chicago Manual of Style (17th edition)

mco1242316433
738
© 2009, all rights reserved.
This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.