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Development and Invitro Characterization of Erythrosine nanoparticles for Chronic Sinusitis using Phtotdynamic Therapy (PDT)

Garapati, Chandrasekhar
http://rave.ohiolink.edu/etdc/view?acc_num=mco1404579514

Abstract Details

2014, Master of Science (MS), University of Toledo, Pharmaceutical Sciences (Industrial Pharmacy).
Antimicrobial therapy for sinusitis has been shown to reduce or eliminate pathologic bacteria associated with rhinosinusitis and improve the symptoms associated with the disease. However, the continuing rise in antibiotic resistance, the ongoing problem with patient compliance, and the intrinsic difficulty in eradication of biofilms complicates antibiotic therapy. The introduction of photodynamic antimicrobial therapy (PAT) using erythrosine, a photosensitizer, could eliminate the bacteria without inducing antibiotic resistance or even requiring daily dosing. In the present study, erythrosine nanoparticles were prepared using poly-lactic-co-glycolic acid (PLGA) and evaluated for their potential in PAT against Staphylococcus aureus bio¿lms. PLGA nanoparticles of erythrosine were prepared by nanoprecipitation technique. Erythrosine nanoparticles were characterized for size, zeta potential, morphology and in vitro release. Qualitative and quantitative uptake studies of erythrosine nanoparticles were carried out in S. aureus biofilms. Photodynamic inactivation of S. aureus biofilms in the presence of erythrosine nanoparticles was investigated by colony forming unit assay. Nanoprecipitation technique resulted in nanoparticles with a mean diameter of 385nm and zeta potential of -9.36 mV. Erythrosine was slowly released from nanoparticles over a period of 120 h. The qualitative study using flow cytometry showed the ability of S. aureus cells to internalize erythrosine nanoparticles. Moreover, erythrosine nanoparticles exhibited a significantly higher uptake and antimicrobial efficacy compared to pure drug in S. aureus biofilms. In conclusion, erythrosine-loaded PLGA nanoparticles can be a potential long term drug delivery system for PAT and are useful for the eradication of S.aureus biofilms.
Sai Hanuman Sagar Boddu (Advisor)
Pharmaceuticals; Pharmacy Sciences

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Citations

  • Garapati, C. (2014). Development and Invitro Characterization of Erythrosine nanoparticles for Chronic Sinusitis using Phtotdynamic Therapy (PDT) [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1404579514

    APA Style (7th edition)

  • Garapati, Chandrasekhar. Development and Invitro Characterization of Erythrosine nanoparticles for Chronic Sinusitis using Phtotdynamic Therapy (PDT). 2014. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1404579514.

    MLA Style (8th edition)

  • Garapati, Chandrasekhar. "Development and Invitro Characterization of Erythrosine nanoparticles for Chronic Sinusitis using Phtotdynamic Therapy (PDT)." Master's thesis, University of Toledo, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=mco1404579514

    Chicago Manual of Style (17th edition)

mco1404579514
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© 2014, all rights reserved.
This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.