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Thesis Kush Patel.pdf (2.13 MB)

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Preparation and Characterization of Insulin loaded Calcium Alginate Nanoparticles

Patel, Kush
http://rave.ohiolink.edu/etdc/view?acc_num=mco1404662134

Abstract Details

2014, Master of Science (MS), University of Toledo, Pharmaceutical Sciences (Industrial Pharmacy).
The purpose of this research work was to develop insulin loaded calcium alginate nanoparticles (CANp) for oral delivery of insulin. The preparation of Insulin-loaded calcium alginate nanoparticles was performed by a novel and efficient method, interfacial cross-linking in nanoemulsions (IFaCLiNE) developed in our research lab. The prepared nanoparticles were evaluated by particle characterization and surface charge measurements. Subsequently, human recombinant, insulin was loaded into CANp. Differential scanning calorimetry (DSC) was used to evaluate the thermal properties. The quantitative determination of insulin in CANp was done via an ELISA assay and in vitro drug release studies. A reversed phase C18 HPLC column with fixed compositions of 0.2 M sodium sulfate buffer and acetonitrile as mobile phases was used in the HPLC analysis of insulin. The insulin peak retention time was observed to be 7.2 minutes. To further identify the stability of insulin in CaNP the qualitative determination of insulin was made through LC-MS tandem mass spectrometry. The determination of precursor ions was achieved through mass spectroscopy (MS) and the insulin peak detection using liquid chromatography.
Jerry Nesamony (Committee Chair)
Pharmaceuticals

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Citations

  • Patel, K. (2014). Preparation and Characterization of Insulin loaded Calcium Alginate Nanoparticles [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1404662134

    APA Style (7th edition)

  • Patel, Kush. Preparation and Characterization of Insulin loaded Calcium Alginate Nanoparticles. 2014. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1404662134.

    MLA Style (8th edition)

  • Patel, Kush. "Preparation and Characterization of Insulin loaded Calcium Alginate Nanoparticles." Master's thesis, University of Toledo, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=mco1404662134

    Chicago Manual of Style (17th edition)

mco1404662134
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This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.