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A Thesis Entitled The Evaluation of Neurotrophic Factor’s Ability to Prevent Induced Cell Death in a PC12 Cell Based Huntington’s Disease Model

Wisner, Alexander S.
http://rave.ohiolink.edu/etdc/view?acc_num=mco1438346174

Abstract Details

2015, Master of Science (MS), University of Toledo, Pharmaceutical Sciences (Pharmacology/Toxicology).
Huntington’s disease (HD) is caused by a genetic mutation of the IT-15 gene resulting in the expansion of the trinucleotide CAG in the huntingtin protein (MacDonald et al., 2003). The mechanism(s) by which this expanded protein causes the disease remains unknown, and there is currently no treatment to halt or prevent development of the disease. An assay was designed to measure the ability of three neurotrophic peptides (Colivelin, D-SAL, D-NAP) to protect cultured PC12 cells from death caused by an expanded form of human huntingtin exon 1. Each neurotrophic peptide was tested at six concentrations for its ability to protect cells against huntingtin-induced cell death. Cell viability was determined by an LDH activity assay that measured the change in absorbance over time. Neurotrophic peptides Colivelin and D-SAL promoted cell viability in both treatment types tested whereas D-NAP only promoted cell viability in the twenty-four hour pre-incubation treatment type.
Youssef Sari, Dr. (Committee Co-Chair)
Frederick Williams, Dr. (Committee Co-Chair)
Caren Steinmiller, Dr. (Committee Member)
Kathryn Eisenmann, Dr. (Committee Member)
Pharmacology
Huntingtin; neurotrophic; huntington disease; PC12; htt; mhtt; Colivelin; D-NAP; D-SAL

Recommended Citations

Citations

  • Wisner, A. S. (2015). A Thesis Entitled The Evaluation of Neurotrophic Factor’s Ability to Prevent Induced Cell Death in a PC12 Cell Based Huntington’s Disease Model [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1438346174

    APA Style (7th edition)

  • Wisner, Alexander. A Thesis Entitled The Evaluation of Neurotrophic Factor’s Ability to Prevent Induced Cell Death in a PC12 Cell Based Huntington’s Disease Model . 2015. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1438346174.

    MLA Style (8th edition)

  • Wisner, Alexander. "A Thesis Entitled The Evaluation of Neurotrophic Factor’s Ability to Prevent Induced Cell Death in a PC12 Cell Based Huntington’s Disease Model ." Master's thesis, University of Toledo, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=mco1438346174

    Chicago Manual of Style (17th edition)

mco1438346174
304
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This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.