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Development and Preliminary In vitro Evaluation of Nanomicelles Laden In situ Gel of Dexamethasone for Ophthalmic Delivery

Chowdhury, Pallabita

Abstract Details

2015, Master of Science (MS), University of Toledo, College of Pharmacy.
In our previous work we developed and characterized 0.1% dexamethasone mixed nanomicelles (DMN). DMN were prepared using surfactants polyoxyl 40 stearate (P40S) and polysorbate 80 (P80), which are approved by the FDA for ocular use. The present study builds on the previous work by developing and evaluating nanomicelles laden in situ gel of 0.1% dexamethasone (DMN-ISG) with potential for treating anterior segment eye inflammations. DMN-ISG was prepared by mixing the basic 2X formulation of DMN with appropriate concentrations of gellan gum, mannitol, benzododecinium bromide and tromethamine. DMN-ISG was characterized for gelation, viscosity, transparency, morphology using Transmission Electron Microscopy (TEM), thermoanalysis using Differential Scanning Calorimetry (DSC), in vitro drug release and sterility. DMN prepared with an optimized composition of P40S/P80=7/3 by weight were used in the preparation of DMN-ISG. TEM image of DMN-ISG showed the presence of dexamethasone nanomicelles in the size range between 20-40 nm entrapped in the gel structure. More than 50% of the drug was released from DMN-ISG in the first few hours and the remaining drug was released in a sustained manner for up to 30 h. Aseptically prepared DMN-ISG formulation remained sterile for up to 14 days. The preliminary findings of our investigation suggest that DMN-ISG has the potential for use in treating anterior segment eye inflammations. Further in vivo evaluation is warranted.
Sai Boddu, Dr. (Advisor)
Kenneth Alexander, Dr. (Committee Member)
Caren Steinmiller, Dr. (Committee Member)

Recommended Citations

Citations

  • Chowdhury, P. (2015). Development and Preliminary In vitro Evaluation of Nanomicelles Laden In situ Gel of Dexamethasone for Ophthalmic Delivery [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1438857060

    APA Style (7th edition)

  • Chowdhury, Pallabita. Development and Preliminary In vitro Evaluation of Nanomicelles Laden In situ Gel of Dexamethasone for Ophthalmic Delivery. 2015. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1438857060.

    MLA Style (8th edition)

  • Chowdhury, Pallabita. "Development and Preliminary In vitro Evaluation of Nanomicelles Laden In situ Gel of Dexamethasone for Ophthalmic Delivery." Master's thesis, University of Toledo, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=mco1438857060

    Chicago Manual of Style (17th edition)