Skip to Main Content
 

Global Search Box

 
 
 

ETD Abstract Container

Abstract Header

Tanshinone IIA Inhibits VEGF Secretion and HIF-1a Expression in Cultured Human Retinal Pigment Epithelial Cells under Hypoxia

Alzhrani, Rami Mohammed

Abstract Details

2016, Master of Science in Pharmaceutical Science (MSP), University of Toledo, Pharmaceutical Sciences (Industrial Pharmacy).
Abstract: Purpose: The current work intends to study the activity of tanshinone IIA on secretion of VEGF and expression of HIF-1a in human retinal pigment epithelial cells (ARPE-19 cells) under hypoxic condition. Methods: The cytotoxicity of Tanshinone IIA was tested in ARPE-19 and bovine corneal endothelial cells by MTT assay. ARPE-19 cells were incubated with different concentrations of cobalt chloride (100, 150, and 200 µM) for 12 hours and levels of expressed HIF-1a and secreted VEGF were quantified through Western blot and ELISA, respectively. Further, ARPE-19 cells were pretreated for one hour with different concentrations of Tanshinone IIA (5, 10, 15 and 18 µM). After one hour, the cells were subjected to hypoxic condition using 150 µM cobalt chloride for 12 hours in the presence and absence of Tanshinone IIA. The cells were then harvested and the secreted VEGF and expressed HIF-1a was studied. Results: Tanshinone IIA at concentrations 5, 10, 15 and 18 µM did not show cytotoxicity in both ARPE-19 and bovine corneal endothelial cells. Chemical hypoxia induced by cobalt chloride caused a significant increase in VEGF level in a dose dependent manner and HIF-1a expression peaked at 150 µM. Based on the data, cobalt chloride concentration was maintained at 150 µM for further studies. Tanshinone IIA decreased the level of HIF-1a and VEGF secretion in a dose dependent manner under hypoxic condition. Conclusion: This study is the first report on the effects of Tanshinone IIA on VEGF secretion and HIF-1a level in ARPE-19 cells. Tanshinone IIA could be a potential new treatment option for wet AMD.
Sai Boddu (Advisor)
Zahoor Shah (Committee Member)
Jerry Nesamony (Committee Member)
80 p.

Recommended Citations

Citations

  • Alzhrani, R. M. (2016). Tanshinone IIA Inhibits VEGF Secretion and HIF-1a Expression in Cultured Human Retinal Pigment Epithelial Cells under Hypoxia [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1468407344

    APA Style (7th edition)

  • Alzhrani, Rami. Tanshinone IIA Inhibits VEGF Secretion and HIF-1a Expression in Cultured Human Retinal Pigment Epithelial Cells under Hypoxia. 2016. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1468407344.

    MLA Style (8th edition)

  • Alzhrani, Rami. "Tanshinone IIA Inhibits VEGF Secretion and HIF-1a Expression in Cultured Human Retinal Pigment Epithelial Cells under Hypoxia." Master's thesis, University of Toledo, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=mco1468407344

    Chicago Manual of Style (17th edition)