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Comparison of Electrospun and Solvent Cast PLA/PVA Inserts as Potential Ocular Drug Delivery Vehicles

Bhattarai, Rajan Sharma, Bhattarai
http://orcid.org/0000-0001-7779-2294
http://rave.ohiolink.edu/etdc/view?acc_num=mco1469787337

Abstract Details

2016, Master of Science in Pharmaceutical Science (MSP), University of Toledo, Pharmaceutical Sciences (Industrial Pharmacy).
Purpose: The purpose of this work was to develop, characterize and compare electrospun nanofiber inserts (ENIs) and solvent cast polymeric inserts for ocular drug delivery. Methods: ENI and solvent cast inserts (SCIs) of dexamethasone were fabricated using a blend of poly-lactic acid (PLA) and poly-vinyl alcohol (PVA). Inserts contained 1%, 5% and 10% dexamethasone (by weight). Inserts were characterized for morphology, thickness, pH in simulated tear fluid (STF, pH 7.4), drug content, thermal analysis, infrared (IR) spectroscopy, in vitro drug release, dimethylformamide (DMF) and chloroform content and cytotoxicity in cultured bovine corneal endothelial cells. The inserts were sterilized by UV radiation for 10 minutes and tested for sterility using plate and direct inoculation techniques. Results: The thickness of 1%, 5%, and 10% dexamethasone-loaded ENIs were found to be 50 µm, 62.5 µm, and 93.3 µm, respectively with good folding endurance. Inserts prepared using the solvent casting technique were brittle with thickness values greater than 200 µm. The release rate of dexamethasone from 1%, 5% and 10% ENIs were found to be 0.62 µg/h, 1.46 µg/h, and 2.30 µg/h respectively, while those from solvent cast technique iv were erratic. A sustained release of dexamethasone was observed from 10% inserts for up to 36 hours. The quantities of DMF in ENIs and SCIs were found to be 0.007% w/w and 0.123% w/w, respectively. The chloroform content in both the inserts was below the detection limit. In addition, the inserts were sterilized by UV radiation and tested for sterility by direct inoculation in tubes and plate inoculation method in a petri dish. No cytotoxicity was observed from ENIs in cultured bovine corneal endothelial cells for up to 24 hours. Conclusion: Based on the results obtained, we conclude that ENIs are better than inserts obtained by solvent casting technique and could be utilized as a potential delivery system for treating anterior segment ocular diseases.
Sai Hanuman Sagar Boddu, PhD. (Committee Chair)
Jerry Nesamony, PhD. (Committee Member)
Sarit Bhaduri, PhD. (Committee Member)
117 p.
Pharmaceuticals; Pharmacy Sciences
Electrospinning, solvent casting, dexamethasone, toxicity

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Citations

  • Bhattarai, Bhattarai, R. S. (2016). Comparison of Electrospun and Solvent Cast PLA/PVA Inserts as Potential Ocular Drug Delivery Vehicles [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1469787337

    APA Style (7th edition)

  • Bhattarai, Bhattarai, Rajan Sharma. Comparison of Electrospun and Solvent Cast PLA/PVA Inserts as Potential Ocular Drug Delivery Vehicles. 2016. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1469787337.

    MLA Style (8th edition)

  • Bhattarai, Bhattarai, Rajan Sharma. "Comparison of Electrospun and Solvent Cast PLA/PVA Inserts as Potential Ocular Drug Delivery Vehicles." Master's thesis, University of Toledo, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=mco1469787337

    Chicago Manual of Style (17th edition)

mco1469787337
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This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.