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Molecular Cloning, Expression, and Characterization of A Novel Zebrafish Cytosolic Sulfotransferase, SULT5A1

Almarghalani, Daniyah Abduljalil
http://rave.ohiolink.edu/etdc/view?acc_num=mco1470097207

Abstract Details

2016, Master of Science in Pharmaceutical Science (MSP), University of Toledo, Pharmaceutical Sciences (Pharmacology/Toxicology).
Sulfotransferase enzymes (SULTs) are responsible for phase II detoxification of xenobiotics as well as regulation of many endogenous compounds, including thyroid/ steroid hormones, bile acids, and catecholamine neurotransmitters. In recent years, zebrafish is emerging as an important animal model for drug metabolism research. This study is part of an overall effort to establish the zebrafish as a model for studying drug sulfation. By searching the GenBank database, the last remaining zebrafish sequence encoding a putative SULT, designated SULT5A1, was identified. Zebrafish SULT5A1 was subsequently cloned, expressed, purified, and characterized. Substrate specificity of zebrafish SULT5A1 was analyzed using a panel of more than 147 xenobiotics, endogenous compound, bile acids, and commercially available bile alcohols. SULT5A1 showed strong sulfating activity toward bile acid and bile alcohol compounds, including 5a-cyprinol, 5ß-cyprinol, 5ß- scymnol, 5ß-cholestantriol, PZ, and 5a-lithocholic acid. It also exhibited significant activity toward endogenous compound, including DHEA and pregnenolone. However, SULT5A1 showed no activity toward xenobiotics. pH dependence and kinetic studies were performed using zebrafish SULT5A1 with 5a-cyprinol, 5a-petromyzonol (PZ), DHEA, and pregnenolone as substrates.
Ming-Cheh Liu (Committee Chair)
Youssef Sari (Committee Member)
Zahoor Shah (Committee Member)
Caren Steinmiller (Other)
48 p.
Pharmaceuticals; Pharmacology; Pharmacy Sciences
Sulfotransferase; SULT; Sulfation; Zebrafish; Bile acid; Bile alcohol; DHEA, Pregnenolone

Recommended Citations

Citations

  • Almarghalani, D. A. (2016). Molecular Cloning, Expression, and Characterization of A Novel Zebrafish Cytosolic Sulfotransferase, SULT5A1 [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1470097207

    APA Style (7th edition)

  • Almarghalani, Daniyah. Molecular Cloning, Expression, and Characterization of A Novel Zebrafish Cytosolic Sulfotransferase, SULT5A1. 2016. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1470097207.

    MLA Style (8th edition)

  • Almarghalani, Daniyah. "Molecular Cloning, Expression, and Characterization of A Novel Zebrafish Cytosolic Sulfotransferase, SULT5A1." Master's thesis, University of Toledo, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=mco1470097207

    Chicago Manual of Style (17th edition)

mco1470097207
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© 2016, all rights reserved.
This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.