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Assessment of the Percutaneous Absorption of ABH PLO Gel Across Porcine Ear Skin

Abstract Details

2018, Master of Science in Pharmaceutical Science (MSP), University of Toledo, Pharmaceutical Sciences (Industrial Pharmacy).
The research’s overall objective is to determine the percutaneous absorption of ABH PLO gel and determine its suitability for topical application. PLO is one of the most popular formularies among the hospice patients and often used anecdotally for treating chemotherapy-induced nausea and vomiting. Lorazepam (Ativan®), diphenhydramine (Benadryl®), haloperidol (Haldol®) (ABH) topical gel is used widely in relieving chemotherapy-induced nausea and vomiting (CINV) for terminally ill hospice patients, because of its presumed efficacy and its low cost. This work aims (a) to develop and characterize the ABH PLO gel containing IPP/lecithin and Pluronic F127. (b) to develop and validate an HPLC method for the simultaneous analysis of ABH drugs, (c) to study the percutaneous absorption of ABH PLO gel through the porcine ear skin, and (d) to determine the theoretical steady state concentration (Css) of ABH drugs from the flux values. ABH PLO gel was characterized for pH, morphology, stability, viscosity, drug content, and thermal analysis using differential scanning calorimetry (DSC). A simple, sensitive and robust HPLC method was developed and validated for simultaneous analysis of ABH drugs. The percutaneous absorption of lorazepam, diphenhydramine, haloperidol from ABH PLO gel was carried out using Franz cells across the pig ear skin and Strat-M membrane. pH of the ABH PLO gel was found to be 5.66±0.13. The retention times of diphenhydramine, haloperidol and lorazepam was found to be 5.2, 7.8, and 18.9 minutes, respectively. Accuracy and intraday precision were satisfactory with RSD < 2%. ABH PLO gel was found to be stable for up to 30 days. Theoretical steady state concentration (Css) of diphenhydramine, haloperidol and lorazepam calculated from percutaneous study were found to be 1.6 ng/ml, 0.13 ng/ml and 2.30 ng/ml, respectively. The theoretical (Css) for ABH drugs were much lower than the required therapeutic concentrations for antiemetic activity to relieve CINV. From this study, it was concluded that the ABH PLO gel may not be effective in treating CINV following topical application. Use of better permeation enhancers/transdermal strategies is needed to achieve required permeation of ABH drugs across the skin.
Sai HanumanSagar Boddu (Committee Chair)
Jerry Nesamony (Committee Member)
Caren Steinmiller (Committee Member)

Recommended Citations

Citations

  • Dahal, A. (2018). Assessment of the Percutaneous Absorption of ABH PLO Gel Across Porcine Ear Skin [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1533321469656146

    APA Style (7th edition)

  • Dahal, Amit. Assessment of the Percutaneous Absorption of ABH PLO Gel Across Porcine Ear Skin. 2018. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1533321469656146.

    MLA Style (8th edition)

  • Dahal, Amit. "Assessment of the Percutaneous Absorption of ABH PLO Gel Across Porcine Ear Skin." Master's thesis, University of Toledo, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=mco1533321469656146

    Chicago Manual of Style (17th edition)