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Phylogenetic Analysis of the Heterocystous Cyanobacteria as Assessed by 16S and 23S Ribosomal RNA

Kenyon, Kyle Christopher
http://rave.ohiolink.edu/etdc/view?acc_num=miami1060270882

Abstract Details

2003, Master of Science, Miami University, Botany.
This study used 16S and 23S rRNA sequence data for phylogenetic analysis of the heterocystous cyanobacteria. Twenty-eight strains of cyanobacteria were cultured in the laboratory; DNA was extracted; the 16S and 23S ribosomal RNA genes amplified by PCR, cloned, and sequenced. Parsimony, distance and maximum likelihood analysis of the 16S and 23S data supports the monophyly of the heterocystous cyanobacteria. Subsection V cyanobacteria are also supported as a monophyletic clade, embedded within Subsection IV. Members of the genera Calothrix, Tolypothrix and Rivularia are paraphyletic and their relationship is not clearly defined. The Nostoc and Anabaena are not monophyletic or sister to one another. Based on these analyses Anabaena PCC7120 should be reassigned as a Nostoc and PCC73102 Nostoc is not well supported as a type strain for the genus. Nodularia and Cylindrospermum strains are well-supported clades. Two distinct copies of the 23S rRNA were detected in several strains.
Susan Barnum (Advisor)
51 p.
Phylogeny; Heterocyst; Heterocystous; Cyanobacteria; 16S ribosomal RNA; 23S ribosomal RNA; Subsection IV; Subsection V; Blue-green algae; systematics

Recommended Citations

Citations

  • Kenyon, K. C. (2003). Phylogenetic Analysis of the Heterocystous Cyanobacteria as Assessed by 16S and 23S Ribosomal RNA [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1060270882

    APA Style (7th edition)

  • Kenyon, Kyle. Phylogenetic Analysis of the Heterocystous Cyanobacteria as Assessed by 16S and 23S Ribosomal RNA. 2003. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1060270882.

    MLA Style (8th edition)

  • Kenyon, Kyle. "Phylogenetic Analysis of the Heterocystous Cyanobacteria as Assessed by 16S and 23S Ribosomal RNA." Master's thesis, Miami University, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=miami1060270882

    Chicago Manual of Style (17th edition)

miami1060270882
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© 2003, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.