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CHARACTERIZATION OF THE HUMAN Na+, K+-ATPASE ALPHA 4 ISOFORM

Hlivko, Jonathan Thomas

Abstract Details

2003, Master of Science, Miami University, Zoology.
Four Na+, K+-ATPase alpha isoforms have been identified and characterized in the rat. In humans, three isoforms have been characterized and evidence has been provided suggesting the existence of a fourth alpha isoform. The goal of this study was to isolate and characterize the fourth human alpha isoform. This was accomplished by cloning the cDNA from human testis libraries and expressing it in HeLa cells. Sequence analysis demonstrates that the deduced amino acid sequence shares high homology with the other three human alpha isoforms and the rat alpha 4 isoform. Using an alpha 4 specific antibody, a protein ~100 kDa was identified through western blotting and it was localized to the flagellum of the sperm by immunohistochemistry. These results confirm the existence of the human alpha 4 Na+, K+-ATPase isoform and its localization to sperm suggest that the human alpha 4 isoform plays a role in the function human sperm.
Paul James (Advisor)
82 p.

Recommended Citations

Citations

  • Hlivko, J. T. (2003). CHARACTERIZATION OF THE HUMAN Na+, K+-ATPASE ALPHA 4 ISOFORM [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1070628755

    APA Style (7th edition)

  • Hlivko, Jonathan. CHARACTERIZATION OF THE HUMAN Na+, K+-ATPASE ALPHA 4 ISOFORM. 2003. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1070628755.

    MLA Style (8th edition)

  • Hlivko, Jonathan. "CHARACTERIZATION OF THE HUMAN Na+, K+-ATPASE ALPHA 4 ISOFORM." Master's thesis, Miami University, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=miami1070628755

    Chicago Manual of Style (17th edition)