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miami1089232925.pdf (712.2 KB)
ETD Abstract Container
Abstract Header
Oxidation of Polymeric Polyphenols (Tannins) in Biologically Relevent Systems
Author Info
Chen, Yumin
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=miami1089232925
Abstract Details
Year and Degree
2004, Doctor of Philosophy, Miami University, Chemistry and Biochemistry.
Abstract
Reactive oxygen species (ROS) are produced in human metabolic processes and uncontrolled ROS are detrimental to human health.
In vitro
chemical assays show that dietary tannins have potent ROS scavenging activity. My research is designed to use model systems to reveal interactions between tannins and factors (pH and protein) found in the human digestive tract where tannins work as biological antioxidants. Pentagalloyl glucose (PGG), bovine serum albumin (BSA), and NaIO
4
were chosen as the model tannin, protein and oxidant, respectively. PGG was prepared from tannic acids via a methanolysis reaction. [
14
C]PGG was synthesized from [U-
14
C]-D-glucopyranose and tri-
O
-benzylgallic acid as a radiochemical tracer. When PGG was oxidized by NaIO
4
, the formation of different oxidation products was controlled by the reaction pH. PGG oxidation produced an o-semiquinone radical intermediate, which formed polymeric products at low pH (e.g. pH 2.1). The
o
-semiquinone radical ionized at pH > 5 and was labile to being further oxidized to
o
-quinone. Reaction pH similarly affected the formation of oxidation products of epicatechin16 (4→8) catechin (procyanidin) or epigallocatechin gallate (EGCG), suggesting that these three different tannins followed the same oxidation mechanism. When PGG was oxidized in the presence of BSA at various pH values, BSA promoted the formation of quinone but inhibited the formation of polymeric products. In addition to affecting PGG oxidation, BSA interacted with PGG under oxidizing conditions and formed oxidized PGG-BSA complexes. A radiochemical method was devised to quantitate oxidized PGG-BSA complexes. The complexes were treated with sodium dodecyl sulfate (SDS) to remove any non-covalently bound PGG and were co-precipitated with BSA by trichloroacetic acid (TCA) for radiochemical assessment. The molar ratio of PGG and BSA determined the solubility of oxidized PGG-BSA complexes. Soluble complexes were formed at low molar ratios (e.g. PGG/BSA=1). At high molar ratios (e.g. PGG/BSA=22), soluble complexes were rapidly converted to insoluble complexes. Besides NaIO
4
, 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS
•+
) and 2,2’-azobis(isobutyramidine) dihydrochloride (AAPH) were tested as oxidants. It was found that NaIO
4
and ABTS
•+
induced the formation of oxidized PGG-BSA complexes more rapidly than AAPH.
Committee
Ann Hagerman (Advisor)
Robert Minto (Other)
Christopher Makaroff (Other)
Michael Crowder (Other)
Helaine Alessiio (Other)
Pages
132 p.
Subject Headings
Chemistry, Biochemistry
Keywords
Polyphenol
;
Tannins
;
Hydrolyzable tannins
;
Tannin-protein interaction
;
Polyphenol-protein interaction
;
Polyphenol oxidation
;
Tannin oxidation
;
Antioxidant
;
Pentagalloyl glucose
;
Oxidized tannin-protein complexes
;
Oxidized polyphenol-protein comp
Recommended Citations
Refworks
EndNote
RIS
Mendeley
Citations
Chen, Y. (2004).
Oxidation of Polymeric Polyphenols (Tannins) in Biologically Relevent Systems
[Doctoral dissertation, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1089232925
APA Style (7th edition)
Chen, Yumin.
Oxidation of Polymeric Polyphenols (Tannins) in Biologically Relevent Systems.
2004. Miami University, Doctoral dissertation.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=miami1089232925.
MLA Style (8th edition)
Chen, Yumin. "Oxidation of Polymeric Polyphenols (Tannins) in Biologically Relevent Systems." Doctoral dissertation, Miami University, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=miami1089232925
Chicago Manual of Style (17th edition)
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Document number:
miami1089232925
Download Count:
2,230
Copyright Info
© 2004, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.