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ALTERED GENE EXPRESSION: A MECHANISM OF REPRODUCTIVE TOXICITY IN ZEBRAFISH (DANIO RERIO) EXPOSED TO BENZO[a]PYRENE

Hoffmann, Jennifer
http://rave.ohiolink.edu/etdc/view?acc_num=miami1092767527

Abstract Details

2004, Doctor of Philosophy, Miami University, Zoology.
Although reductions in 17ß-estradiol and vitellogenin concentrations have been consistently observed in fish exposed to polycyclic aromatic hydrocarbons (PAHs), an understanding of the mechanism behind these changes has yet to be fully elucidated. The overall objective of the present research was to gain a better understanding of the effect of PAHs on transcription of genes involved in reproduction. The first objective of this research was to characterize the expression of follicle stimulating hormone receptor (FSH-R) and luteinizing hormone receptor (LH-R) and four key steroidogenic enzymes (P450-side chain cleavage (P450scc), P450 17α-Hydroxylase/17-20 lyase (P450c17), 20β-Hydroxysteroid Dehydrogenase (20β-HSD), P450-aromatase (P450arom)) in oocytes and whole ovaries at different stages of development in zebrafish to gain a better understanding of basic transcriptional regulation of ovarian development and maturation. All genes examined were differentially expressed in pre-, early, mid, and late-vitellogenic oocytes. In contrast, only LH-R was differentially expressed at 0, 48, and 96 hours after spawning. The results of the first experiment provided evidence that transcription of gonadotropin receptors and steroidogenic enzymes may be involved in regulating ovarian development and maturation. Additional objectives of this project were 1) to determine if exposure to waterborne benzo[a]pyrene (B[a]P) (0, 1.5, or 3.0 µg/L) for 61 days affected reproduction in zebrafish and 2) to determine if waterborne exposure to B[a]P affected transcription of gonadotropins, steroidogenic enzymes, activin, vitellogenin, or estrogen receptor β. A 50% and 48% reduction in total egg output was observed in the 1.5 µg/L and 3.0 µ/L treatments as compared to controls, respectively. Although not significant, there was a trend towards a decrease in the number of females that spawned following exposure to 3 µg B[a]P/L. Cytochrome P4501A1 (CYP1A1) mRNA was significantly increased in heads of B[a]P-exposed zebrafish whereas it was not elevated in livers of B[a]P-exposed fish. An increase in P450aromB, vitellogenin, and 20β-HSD mRNA occurred in fish exposed to 3.0 μg B[a]P/L as compared to the controls. The results from this study demonstrate that reproduction in zebrafish is affected by waterborne exposure to B[a]P and that transcription of P450aromB, vitellogenin, and 20β-HSD may be one mechanism of B[a]P-induced reproductive toxicity. U
James Oris (Advisor)
138 p.
Zebrafish; Benzo[a]pyrene; ovary; steroidogenic enzymes; mRNA

Recommended Citations

Citations

  • Hoffmann, J. (2004). ALTERED GENE EXPRESSION: A MECHANISM OF REPRODUCTIVE TOXICITY IN ZEBRAFISH (DANIO RERIO) EXPOSED TO BENZO[a]PYRENE [Doctoral dissertation, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1092767527

    APA Style (7th edition)

  • Hoffmann, Jennifer. ALTERED GENE EXPRESSION: A MECHANISM OF REPRODUCTIVE TOXICITY IN ZEBRAFISH (DANIO RERIO) EXPOSED TO BENZO[a]PYRENE. 2004. Miami University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1092767527.

    MLA Style (8th edition)

  • Hoffmann, Jennifer. "ALTERED GENE EXPRESSION: A MECHANISM OF REPRODUCTIVE TOXICITY IN ZEBRAFISH (DANIO RERIO) EXPOSED TO BENZO[a]PYRENE." Doctoral dissertation, Miami University, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=miami1092767527

    Chicago Manual of Style (17th edition)

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This open access ETD is published by Miami University and OhioLINK.