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Investigation of Burkholderia cepacia Virulence

Mykrantz, Hallie B
http://rave.ohiolink.edu/etdc/view?acc_num=miami1114116170

Abstract Details

2005, Master of Science, Miami University, Microbiology.
Burkholderia cepacia is an opportunistic pathogen that causes a variety of infections. Individuals with cystic fibrosis, a lethal autosomal-recessive disease, contract nosocomial infections of B. cepacia that are challenging to treat due to the organism’s resistance to multiple antibiotics. The mechanisms used by the bacterium to cause these infections are currently unknown and, therefore, alternative methods of treatment or prevention are difficult to establish. This project examined several B. cepacia strains and avirulent B. cepacia transconjugants to investigate potential virulence mechanisms. The data suggest that the extracellular enzymes protease, lipase, and phospholipase C do not significantly contribute to the pathogenicity of the organism, at least in the Caenorhabditis elegans model we used. Infection, not intoxication, by B. cepacia of C. elegans was responsible, but not necessarily sufficient for the nematocidal activities of all the strains studied. The results of these experiments also support the suitability of C. elegans as an in vivo model for studying the virulence factors of B. cepacia.
Anne Morris-Hooke (Advisor)
79 p.
Biology, Microbiology
Caenorhabditis elegans; Burkholderia cepacia; cystic fibrosis; virulence mechanisms; transposon mutagenesis; lipase; protease; phospholipase C

Recommended Citations

Citations

  • Mykrantz, H. B. (2005). Investigation of Burkholderia cepacia Virulence [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1114116170

    APA Style (7th edition)

  • Mykrantz, Hallie. Investigation of Burkholderia cepacia Virulence. 2005. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1114116170.

    MLA Style (8th edition)

  • Mykrantz, Hallie. "Investigation of Burkholderia cepacia Virulence." Master's thesis, Miami University, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=miami1114116170

    Chicago Manual of Style (17th edition)

miami1114116170
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© 2005, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.