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miami1165601341.pdf (2.61 MB)
ETD Abstract Container
Abstract Header
Studies on Cold Resistance in Palms: Analysis of
CBF
-Like Genes
Author Info
Lu, Li
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=miami1165601341
Abstract Details
Year and Degree
2006, Doctor of Philosophy, Miami University, Botany.
Abstract
Cold resistance of plants is an important characteristic that restricts plant distribution, growth and biomass productivity. Cold acclimation is a phenomenon that occurs in some plants as an increase in cold tolerance upon exposure to low, non-freezing temperatures. The
CBF/DREB1
gene family is a small group of transcription factors that play key roles in cold acclimation. The
CBF/DREB1
genes were first identified from the model plant
Arabidopsis
, then from many economically important plant species including grape, tomato, corn, rice, and barley. Overexpression of
CBF/DREB1
genes induces multiple components of cold acclimation and thus increases plant cold tolerance even without cold stimulus. To systematically study the cold resistance mechanism of palms,
CBF/DREB1
orthologs were isolated from various palms, including both cold-resistant and cold-sensitive species. This is the first report of
CBF/DREB1
genes in the Palmae family. Analyses of DNA and putative protein sequences confirmed that the palm CBF/DREB1 has similar structures with known CBF proteins. To further investigate the functions of the palm
CBF/DREB1
genes, the expression patterns of two Rhapidophyllum
hystrix CBF
orthologs,
RhCBF1
and
RhCBF2
, were analyzed with reverse transcription and real-time PCR. The expressions of the two
RhCBFs
were constitutive yet cold-inducible, which provided further evidence of the involvement of these genes in cold acclimation response of palms. The two
RhCBF
genes were transformed into Arabidopsis. The transgenic
Arabidopsis
plants showed increased cold tolerance, typical growth retardation of
CBF
-overexpessing plants, and other multiple
CBF
-related cellular changes. The results indicated that
RhCBFs
had functional similarity with
Arabidopsis CBF
genes also.
Committee
Kenneth Wilson (Advisor)
Pages
90 p.
Keywords
CBF/DREB1
;
cold acclimation
;
cold resistance
;
palm
Recommended Citations
Refworks
EndNote
RIS
Mendeley
Citations
Lu, L. (2006).
Studies on Cold Resistance in Palms: Analysis of
CBF
-Like Genes
[Doctoral dissertation, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1165601341
APA Style (7th edition)
Lu, Li.
Studies on Cold Resistance in Palms: Analysis of
CBF
-Like Genes.
2006. Miami University, Doctoral dissertation.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=miami1165601341.
MLA Style (8th edition)
Lu, Li. "Studies on Cold Resistance in Palms: Analysis of
CBF
-Like Genes." Doctoral dissertation, Miami University, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=miami1165601341
Chicago Manual of Style (17th edition)
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Document number:
miami1165601341
Download Count:
1,053
Copyright Info
© 2006, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.