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Enhanced Cold Tolerance of Diapause-Destined vs Non-Diapause-Destined Larval Stages of the Flesh Fly, Sarcophaga Crassipalpis(Diptera: Sarcophagidae)

Smith, Kent James

Abstract Details

2007, Master of Science, Miami University, Zoology.
The ontogenetic development of cold-hardening associated with diapause was examined in feeding and wandering larval stages of the flesh fly, Sarcophaga crassipalpis. Cold tolerance was greatest for diapause-destined wandering larvae, as 62% of cold-shocked (-9 °C, 2 h) diapause-destined individuals progressed to adult development as compared to only 10% of wandering, non-diapause-destined larvae. The cell viability of Malpighian tubules and fat bodies of cold-shocked diapause-destined wandering larvae were significantly greater (91%, 90%) than in non-diapause-destined groups (64%, 75%). The diapause program influenced the composition of membrane fatty acids (FAs). The dominant unsaturated fatty acid, oleic acid, accounted for only 18% of the total phospholipid FA proportion in diapause-destined groups, down from 36% in non-diapause-destined larvae. This shift in oleic acid, coupled with increases in the principal saturated FA species in diapause-destined larvae, resulted in an index of unsaturation significantly lower (0.57) than in non-diapause-destined larvae (1.08).
Richard Lee (Advisor)
32 p.

Recommended Citations

Citations

  • Smith, K. J. (2007). Enhanced Cold Tolerance of Diapause-Destined vs Non-Diapause-Destined Larval Stages of the Flesh Fly, Sarcophaga Crassipalpis(Diptera: Sarcophagidae) [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1172780125

    APA Style (7th edition)

  • Smith, Kent. Enhanced Cold Tolerance of Diapause-Destined vs Non-Diapause-Destined Larval Stages of the Flesh Fly, Sarcophaga Crassipalpis(Diptera: Sarcophagidae). 2007. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1172780125.

    MLA Style (8th edition)

  • Smith, Kent. "Enhanced Cold Tolerance of Diapause-Destined vs Non-Diapause-Destined Larval Stages of the Flesh Fly, Sarcophaga Crassipalpis(Diptera: Sarcophagidae)." Master's thesis, Miami University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=miami1172780125

    Chicago Manual of Style (17th edition)