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QUANTITATIVE AND QUALITATIVE INVESTIGATIONS INTO URINARY CALCULI USING INFRARED MICROSPECTROSCOPY

Anderson, Jennifer Christine
http://rave.ohiolink.edu/etdc/view?acc_num=miami1173303815

Abstract Details

2007, Doctor of Philosophy, Miami University, Chemistry and Biochemistry.
This dissertation encompasses research focused on both qualitative and quantitative techniques for the analysis of loose urinary calculi (often referred to as renal stones) as well as biopsied tissue sections containing urinary calculi material. Due to high rates of renal calculi misdiagnosis, qualitative techniques that are efficient, accurate, and lack long sample preparation are desperately needed in clinics and hospitals around the world. The techniques presented in this dissertation rely on the use of infrared molecular microspectroscopy and infrared spectral maps for unbiased, fast, and accurate methods of both urinary calculi and tissue analysis. Utilizing infrared reflectance techniques, samples of varying size, shape, thickness, and consistency are easily and quickly analyzed. Additionally, infrared reflectance techniques are non-destructive, allowing the analysis of delicate samples without their contamination or destruction. Infrared spectral maps pin-point and visually differentiate urinary calculi components in a matter of minutes, resulting in an unbiased and accurate diagnosis. Not only are qualitative techniques and results detailed in this dissertation, but quantitative results are included as well. Using infrared attenuated total internal reflectance (ATR) techniques, detection limits of urinary calculi components are investigated. Both concentration detection limits and particle size detection limits are important in the quantification of urinary calculi components. ATR analysis techniques allow the quantitative analysis of particles as small as 10 ìm in size, as well as the analysis of concentrations of urinary calculi components as low as 1% by weight. Finally, an appendix is included outlining the procedure, sample types, and results of an EPA internship lasting 900 hours. The focus of this internship was the analysis of corrosion samples from public and private drinking water systems. The main capacity of this research was confirmatory in nature, using Raman microspectroscopy to confirm or deny the presence of certain lead and copper compounds.
Andre Sommer (Advisor)
251 p.
Chemistry, Analytical
Infrared; Microspectroscopy; Renal Stones; Kidney; Calibration Curve; EPA

Recommended Citations

Citations

  • Anderson, J. C. (2007). QUANTITATIVE AND QUALITATIVE INVESTIGATIONS INTO URINARY CALCULI USING INFRARED MICROSPECTROSCOPY [Doctoral dissertation, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1173303815

    APA Style (7th edition)

  • Anderson, Jennifer. QUANTITATIVE AND QUALITATIVE INVESTIGATIONS INTO URINARY CALCULI USING INFRARED MICROSPECTROSCOPY. 2007. Miami University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1173303815.

    MLA Style (8th edition)

  • Anderson, Jennifer. "QUANTITATIVE AND QUALITATIVE INVESTIGATIONS INTO URINARY CALCULI USING INFRARED MICROSPECTROSCOPY." Doctoral dissertation, Miami University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=miami1173303815

    Chicago Manual of Style (17th edition)

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© 2007, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.