Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


miami1217016926.pdf (454.03 KB)

ETD Abstract Container

Abstract Header

I. Cytotoxicity and anti-inflammatory activity of polyphenolics. II. Polyphenolics in natural soils

Wisman, Kimberly N.
http://rave.ohiolink.edu/etdc/view?acc_num=miami1217016926

Abstract Details

2008, Master of Science, Miami University, Chemistry and Biochemistry.
Phenolic compounds are known for their epidemiological association with reduced risks for certain diseases. We used rabbit peripheral blood mononuclear cells (PBMCs) to examine anti-inflammatory activity and cytotoxicity of nine polyphenolics, representing several classes of tannins. The results from a sandwich ELISA assay for TNF-Α showed that anti-inflammatory activity was only observed with apigenin (EC50 1.0 µg/mL), while other compounds either had no affect on TNF-Α or increased its production. Our study also showed that tannins interfered with the MTT cell viability assay and that chemical reactivity of tannins was changed upon spontaneous oxidation. In addition to ELISA, we began to use real-time RT-PCR to quantify cytokine mRNAs in lysates of cells that were treated with phenolics. Polyphenolic compounds are also of interest in the management of soils. We have begun developing a new method using a resin trap to quantify the input of polyphenolics into soil.
Ann E. Hagerman, PhD (Advisor)
Michael W. Crowder, PhD (Committee Chair)
Neil D. Danielson, PhD (Committee Member)
Christophe A. Makaroff, PhD (Committee Member)
95 p.
Biochemistry; Soil Sciences
Tannins; proanthocyanidins; hydrolysable tannins; polyphenolics; monocytes; PBMC; cell viability; MTT assay; phenolic oxidation; soil; resin trap

Recommended Citations

Citations

  • Wisman, K. N. (2008). I. Cytotoxicity and anti-inflammatory activity of polyphenolics. II. Polyphenolics in natural soils [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1217016926

    APA Style (7th edition)

  • Wisman, Kimberly. I. Cytotoxicity and anti-inflammatory activity of polyphenolics. II. Polyphenolics in natural soils. 2008. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1217016926.

    MLA Style (8th edition)

  • Wisman, Kimberly. "I. Cytotoxicity and anti-inflammatory activity of polyphenolics. II. Polyphenolics in natural soils." Master's thesis, Miami University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=miami1217016926

    Chicago Manual of Style (17th edition)

miami1217016926
549
© 2008, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.