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Methylammonium Formate as a Mobile Phase Modifier for Reversed Phase Liquid Chromatography

Grossman, Shau
http://rave.ohiolink.edu/etdc/view?acc_num=miami1217890628

Abstract Details

2008, Master of Science, Miami University, Chemistry and Biochemistry.
This study focuses on the synthesis and the characterization of the room temperature ionic liquid methylammonium formate (MAF) and its use as an organic solvent replacement for the mobile phase in liquid chromatography. Spectroscopic characterization as well as measurements of physical properties are determined and compared to previously studied alkylammonium formate ionic liquids. Our chromatographic evidence has shown that MAF is a good silanol suppressor resulting in better peak shape and separation efficiency than methanol as a mobile phase modifier when using C18-based silica columns. The preliminary studies using MAF as a mobile phase modifier or additive for pharmaceutical applications using LC-MS methods have shown promising results. The fluorescence studies using cytochrome C as the test compound in five different organic solvents has identified MAF as the least denaturing compared with other solvents including dimethyl sulfoxide, N-methylformamide, methanol, and acetonitrile.
Neil Danielson, PhD (Advisor)
John Hawes, PhD (Committee Member)
Riechel Thomas, PhD (Committee Member)
Shouzhong Zou, PhD (Committee Member)
109 p.
Chemistry
methylammonium formate; room temperature ionic liquid; methylammonium adduct; RPLC; LC-MS; LC/MS/MS; mobile phase modifier; mobile phase additive

Recommended Citations

Citations

  • Grossman, S. (2008). Methylammonium Formate as a Mobile Phase Modifier for Reversed Phase Liquid Chromatography [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1217890628

    APA Style (7th edition)

  • Grossman, Shau. Methylammonium Formate as a Mobile Phase Modifier for Reversed Phase Liquid Chromatography. 2008. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1217890628.

    MLA Style (8th edition)

  • Grossman, Shau. "Methylammonium Formate as a Mobile Phase Modifier for Reversed Phase Liquid Chromatography." Master's thesis, Miami University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=miami1217890628

    Chicago Manual of Style (17th edition)

miami1217890628
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© 2008, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.