Skip to Main Content
 

Global Search Box

 
 
 
 

ETD Abstract Container

Abstract Header

VISUALIZING CHANGES IN THE GENOMIC ORGANIZATION OF RETROTRANSPOSONS IN HELIANTHUS HYBRID SPECIES

Staton, Spencer Evan

Abstract Details

2008, Master of Science, Miami University, Botany.
A specific class of mobile DNA called Class I transposable elements, or retrotransposons, occupy a large fraction of the genomic DNA in the flowering plants (the Angiosperms). Given that there has been a 5.6 to 23.6-fold increase in copy number of Ty3/gypsy retrotransposons in the genomes of two hybrid sunflower species (Helianthus deserticola, H. paradoxus), I investigated whether these elements have invaded new regions of the hybrid genomes, relative to the parents (H. annuus, H. petiolaris). I use Fluorscence in situ Hybridization (FISH) to compare the genomic organization of these elements in the parents and hybrid species. Here I show a significant expansion of Ty3/gypsy retroelements around the centromeres of two hybrid sunflower species and that retrotransposons are dispersed throughout sunflower genomes. This observation is consistent with the idea that plant genomes accommodate large additions of DNA in primarily heterochromatic regions, and that large plant genomes contain many dispersedretroelements.
Richard Moore, PhD (Advisor)
Gorchov Dave, PhD (Committee Member)
Edelmann Richard, PhD (Committee Member)
54 p.

Recommended Citations

Citations

  • Staton, S. E. (2008). VISUALIZING CHANGES IN THE GENOMIC ORGANIZATION OF RETROTRANSPOSONS IN HELIANTHUS HYBRID SPECIES [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1218038514

    APA Style (7th edition)

  • Staton, Spencer. VISUALIZING CHANGES IN THE GENOMIC ORGANIZATION OF RETROTRANSPOSONS IN HELIANTHUS HYBRID SPECIES. 2008. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1218038514.

    MLA Style (8th edition)

  • Staton, Spencer. "VISUALIZING CHANGES IN THE GENOMIC ORGANIZATION OF RETROTRANSPOSONS IN HELIANTHUS HYBRID SPECIES." Master's thesis, Miami University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=miami1218038514

    Chicago Manual of Style (17th edition)