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Programmed cell death and induction of caspase-like protease activity in roots of Glycine max (soybean) in response to flooding stress

Abstract Details

2008, Master of Science, Miami University, Botany.
This study reports the identification Glycine max varieties that form vascular cavities in their primary roots under warm and flooded conditions. Vascular cavity formation is a PCD-mediated process and this study explores the induction of caspase-like proteases, a hallmark of PCD. Injection of caspase inhibitors have resulted in suppression of vascular cavity formation under hypoxia. Substrate cleavage assays using synthetic caspase-specific substrates also show a marked increase in caspase activity after flooding. Further, the caspase like proteases are inhibited only by specific inhibitors and not by general protease inhibitors. Caspase-like proteases are pH sensitive and are effective under acidic pH conditions. Study of in vivo caspase-like protease activity employing peptide-based fluorogenic substrates show their activity in the cytosol of cells in the vascular cylinder of soybean roots. This study is expected to prompt a deeper understanding of hypoxia-induced PCD and add valuable data to existing knowledge of plant PCD
Daniel Gladish (Committee Chair)
Qingshun Li (Committee Member)
Alfredo Huerta (Committee Member)
49 p.

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Citations

  • Sreekanta, S. (2008). Programmed cell death and induction of caspase-like protease activity in roots of Glycine max (soybean) in response to flooding stress [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1218082902

    APA Style (7th edition)

  • Sreekanta, Suma. Programmed cell death and induction of caspase-like protease activity in roots of Glycine max (soybean) in response to flooding stress. 2008. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1218082902.

    MLA Style (8th edition)

  • Sreekanta, Suma. "Programmed cell death and induction of caspase-like protease activity in roots of Glycine max (soybean) in response to flooding stress." Master's thesis, Miami University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=miami1218082902

    Chicago Manual of Style (17th edition)