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Spectroscopic Studies of Proteins in Alkylammonium Formate Ionic Liquids

Wei, Wenjun
http://rave.ohiolink.edu/etdc/view?acc_num=miami1240335504

Abstract Details

2009, Master of Science, Miami University, Chemistry and Biochemistry.
This thesis describes protein structural changes in alkylammonium formate (AAF) ionic liquids. These proteins are dissolved in methylammonium formate (MAF), ethylammonium formate (EAF), and choline formate ionic liquids for study by fluorescence, circular dichroism, and UV-Visible spectroscopy. At room temperature, AAF solutions can maintain the native structure of proteins, such as cytochrome c and lysozyme, in relatively high ionic liquid concentrations (50%-70% AAF/water or AAF/phosphate buffer pH 7.0) compared to similar solutions of the organic solvents, methanol or acetonitrile, with water or buffer. As temperature increases to 80 °C, AAF denatures proteins less than organic solvents. About 1/3 of the enzyme activity of cytochrome c in 80% AAF/water can be maintained as compared to phosphate buffer. This fundamental biophysical information shows that AAFs have potential application as organic or salt solvent replacements for the separation of proteins in their native form by reversed phase or hydrophobic interaction liquid chromatography.
Neil D. Danielson (Advisor)
Andre’J. Sommer (Committee Chair)
Thomas L. Riechel (Committee Member)
Shouzhong Zou (Committee Member)
118 p.
Analytical Chemistry
ionic liquids; alkylammonium formate; MAF; EAF; circular dichroism; fluorescence; cytochrome c; lysozyme; human serum albumin; hexokinase; spectroscopy

Recommended Citations

Citations

  • Wei, W. (2009). Spectroscopic Studies of Proteins in Alkylammonium Formate Ionic Liquids [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1240335504

    APA Style (7th edition)

  • Wei, Wenjun. Spectroscopic Studies of Proteins in Alkylammonium Formate Ionic Liquids. 2009. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1240335504.

    MLA Style (8th edition)

  • Wei, Wenjun. "Spectroscopic Studies of Proteins in Alkylammonium Formate Ionic Liquids." Master's thesis, Miami University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=miami1240335504

    Chicago Manual of Style (17th edition)

miami1240335504
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© 2009, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.