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An Investigation into Quantitative ATR-FT-IR Imaging and Raman Microspectroscopy of Small Mineral Inclusions in Kidney Biopsies

Gulley-Stahl, Heather Jane
http://rave.ohiolink.edu/etdc/view?acc_num=miami1272042834

Abstract Details

2010, Doctor of Philosophy, Miami University, Chemistry and Biochemistry.
This dissertation describes the use of infrared and Raman microspectroscopic methods for the analysis of small mineral inclusions in kidney biopsies and efforts to quantitatively analyze components at the localized mineral/tissue interface. Chapter 1 provides a background on the current state of kidney stone disease research and also describes the instrumental methods utilized. Chapter 2 presents the use of attenuated total internal reflection (ATR) infrared spectroscopy to generate calibration curves for mixtures of powdered kidney stone components. This study demonstrates that reproducible quantitation can be achieved if the particle sizes of the components are small and comparable. ATR imaging was employed to analyze kidney biopsies with small mineral inclusions in Chapter 3. ATR imaging has several benefits over other infrared sampling methods (primarily transflection and transmission) for the analysis of kidney biopsies due to the smaller focused beam size achievable and the reduced optical pathlength that eliminates spectral artifacts. Chapter 4 describes an investigation into small particle analysis with transmission infrared microspectroscopy and ATR imaging. The results of the study show that spectral artifacts are dependent on the particle’s size and shape and it is anticipated that this research will provide a framework for the analysis of particles below the diffraction limit. Chapter 5 presents a comparative study of Raman microspectroscopy and ATR imaging for kidney biopsy analysis. Using both methods in unison allows the investigator to obtain a full spectroscopic picture of the sample. Because Raman interrogates a larger, more uncontrolled sample volume, ATR may be more beneficial for quantitative studies. Chapter 6 summarizes the presented research and discusses future work.
Andre J. Sommer, PhD (Advisor)
Neil D. Danielson, PhD (Committee Chair)
Andrew P. Evan, PhD (Committee Member)
Thomas L. Riechel, PhD (Committee Member)
Lei L. Kerr, PhD (Committee Member)
145 p.
Analytical Chemistry
Quantitative ATR-FT-IR; ATR-FT-IR Microspectroscopic Imaging; Kidney Biopsy Analysis; Mineral Inclusion Analysis; Kidney Stone; Raman Microspectroscopic Mapping

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Citations

  • Gulley-Stahl, H. J. (2010). An Investigation into Quantitative ATR-FT-IR Imaging and Raman Microspectroscopy of Small Mineral Inclusions in Kidney Biopsies [Doctoral dissertation, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1272042834

    APA Style (7th edition)

  • Gulley-Stahl, Heather. An Investigation into Quantitative ATR-FT-IR Imaging and Raman Microspectroscopy of Small Mineral Inclusions in Kidney Biopsies. 2010. Miami University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1272042834.

    MLA Style (8th edition)

  • Gulley-Stahl, Heather. "An Investigation into Quantitative ATR-FT-IR Imaging and Raman Microspectroscopy of Small Mineral Inclusions in Kidney Biopsies." Doctoral dissertation, Miami University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=miami1272042834

    Chicago Manual of Style (17th edition)

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© 2010, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.