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Investigating Cellular DNA Damage Responses Induced During Adenovirus Early Region 4 Mutant Infection and Their Impact on Viral DNA Replication

Clark, Jason P.
http://rave.ohiolink.edu/etdc/view?acc_num=miami1281448618

Abstract Details

2010, Master of Science, Miami University, Microbiology.
DNA double-strand breaks elicit the DNA damage response (DDR) in eukaryotic cells. This response involves the Mre11/Rad50/NBS1 (MRN) complex and activation of DNA damage kinases ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) that phosphorylate proteins involved in cell cycle arrest, DNA repair, and apoptosis. Adenovirus (Ad) is a linear, double-stranded DNA virus, which presents the cell with a genome that resembles a DNA double-strand break. Wild type Ad5 (Ad5) produces proteins that prevent a DDR while Ad early region 4 (E4) mutants lack this ability and therefore induce DDRs in infected cells. This work is aimed at studying the DDR during infection with a particular E4 mutant (H5dl1014) (1014), and analyzing the severe DNA replication defect that this mutant exhibits. From this work I have demonstrated that Ad E4 mutant 1014 infection induces a DDR and that 1014 DNA replication may be regulated through the MRN complex.
Eileen Bridge, Dr (Advisor)
Gary Janssen, Dr (Committee Member)
Xiao-Wen Cheng, Dr (Committee Member)
56 p.
Biology; Cellular Biology; Microbiology; Molecular Biology; Virology
DNA damage response; ATM; MDC1; E4 mutant; adenovirus

Recommended Citations

Citations

  • Clark, J. P. (2010). Investigating Cellular DNA Damage Responses Induced During Adenovirus Early Region 4 Mutant Infection and Their Impact on Viral DNA Replication [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1281448618

    APA Style (7th edition)

  • Clark, Jason. Investigating Cellular DNA Damage Responses Induced During Adenovirus Early Region 4 Mutant Infection and Their Impact on Viral DNA Replication. 2010. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1281448618.

    MLA Style (8th edition)

  • Clark, Jason. "Investigating Cellular DNA Damage Responses Induced During Adenovirus Early Region 4 Mutant Infection and Their Impact on Viral DNA Replication." Master's thesis, Miami University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=miami1281448618

    Chicago Manual of Style (17th edition)

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© 2010, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.