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miami1281640692.pdf (2.79 MB)
ETD Abstract Container
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The Effects of High Hydrostatic Pressures on NADH Conformation
Author Info
Alquist, Erik James
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=miami1281640692
Abstract Details
Year and Degree
2010, Master of Science, Miami University, Physics.
Abstract
Reduced nicotinamide adenine dinucleotide (NADH) plays a central role in cellular metabolism via a redox reaction. Its conformation is physiologically significant as NADH takes a folded conformation when free and an unfolded conformation when protein bound. This study uses fluorescent emission spectroscopy and solvent denaturation to examine pressure’s role in the equilibrium conformation of NADH in solution (10 and 20 μM in MOPS buffer, pH 7.4). Quartz capillary-based high-pressure chambers house samples up to 51 MPa which are excited with a 337 nm nitrogen laser. Using a two-state model, the free energy of unfolding is determined and the volume change of unfolding is measured to be 24.9 ml/mol assuming an Arrhenius relationship. The validities of the two-state model and the selected concentration range are assessed. Results are significant to understanding pressure effects on cellular metabolism and for future studies probing the fluorescent signals of cells under pressure.
Committee
Paul Urayama, PhD (Advisor)
Burçin Bayram, PhD (Committee Member)
Jaeger Herbert, PhD (Committee Member)
Pages
74 p.
Subject Headings
Biophysics
;
Physics
Keywords
NADH
;
High Pressure
;
Conformation
;
Fluorescence
;
Volume change
;
Solvent denaturation
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Citations
Alquist, E. J. (2010).
The Effects of High Hydrostatic Pressures on NADH Conformation
[Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1281640692
APA Style (7th edition)
Alquist, Erik.
The Effects of High Hydrostatic Pressures on NADH Conformation.
2010. Miami University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=miami1281640692.
MLA Style (8th edition)
Alquist, Erik. "The Effects of High Hydrostatic Pressures on NADH Conformation." Master's thesis, Miami University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=miami1281640692
Chicago Manual of Style (17th edition)
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Document number:
miami1281640692
Download Count:
1,225
Copyright Info
© 2010, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.