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Structural and Thermodynamic Characterization of an Alternative Splicing Regulatory Element in HIV-1

Mishler, Clay H. J.
http://rave.ohiolink.edu/etdc/view?acc_num=miami1314287942

Abstract Details

2011, Master of Science, Miami University, Chemistry and Biochemistry.
In order to better understand the alternative splicing mechanism in the human immunodeficiency virus and eventually disrupt it, the structure of the third stem loop in HIV-1 splice site A7, otherwise known as exonic splicing silencer three or SLess3, was determined through solution NMR. The structure was found to form a hairpin loop, exposing important functional groups on the 5’-UAG-3’ motif. Also, the thermodynamic binding determinates of SLess3 to UP1, a deletion mutant of hnRNP A1, were elucidated via isothermal titration calorimetry. UP1 was found to bind to SLess3 in a 1:1 manner with nanomolar affinity.
Blanton Tolbert, PhD (Advisor)
David Tierney, PhD (Committee Chair)
Natosha Finley, PhD (Committee Member)
Carole Dabney-Smith, PhD (Committee Member)
87 p.
Biochemistry
HIV; alternative splicing; RNA

Recommended Citations

Citations

  • Mishler, C. H. J. (2011). Structural and Thermodynamic Characterization of an Alternative Splicing Regulatory Element in HIV-1 [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1314287942

    APA Style (7th edition)

  • Mishler, Clay. Structural and Thermodynamic Characterization of an Alternative Splicing Regulatory Element in HIV-1. 2011. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1314287942.

    MLA Style (8th edition)

  • Mishler, Clay. "Structural and Thermodynamic Characterization of an Alternative Splicing Regulatory Element in HIV-1." Master's thesis, Miami University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=miami1314287942

    Chicago Manual of Style (17th edition)

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© 2011, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.