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Alternative polyadenylation regulates the expression of the light harvesting gene LHCB4.1 in Arabidopsis mutant oxt6

Chen, Jie
http://rave.ohiolink.edu/etdc/view?acc_num=miami1322534400

Abstract Details

2011, Master of Science, Miami University, Botany.
Recent studies revealed differential usage of Alternative Polyadenylation (APA) in Arabidopsis thaliana wild-type and the oxt6 mutant in which the Oxidative Stress Tolerance 6 gene (OXT6) encoding cleavage and polyadenylation specificity factor 30 (CPSF30) is interrupted. In this thesis I confirmed the differential patterns of APA for the gene LHCB4.1, encoding light harvesting complex II subunit, in wild-type versus the oxt6 mutant under either normal conditions or light/temperature stresses by using reverse transcription followed by real time PCR. It suggests the regulatory roles of AtCPSF30 and environmental stresses in the expression level and APA selection of other genes. In addition, leaves of oxt6 plants subjected to dark treatment etiolated faster than that of wild-type under dark treatment. Under dark treatment, LHCB4 protein abundance and its associated complexes were significantly lower in the oxt6 plants than in wild-type as determined by using blue native polyacrylamide gel electrophoresis and western blots. These results suggest that oxt6 mutant affects photosystem productivity and may explain why oxt6 plants have reduced stature.
Qingshun Quinn Li (Advisor)
Chun Liang (Committee Member)
Richard Moore (Committee Member)
42 p.
Botany
oxt6; alternative polyadenylation; LHCB4.1

Recommended Citations

Citations

  • Chen, J. (2011). Alternative polyadenylation regulates the expression of the light harvesting gene LHCB4.1 in Arabidopsis mutant oxt6 [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1322534400

    APA Style (7th edition)

  • Chen, Jie. Alternative polyadenylation regulates the expression of the light harvesting gene LHCB4.1 in Arabidopsis mutant oxt6. 2011. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1322534400.

    MLA Style (8th edition)

  • Chen, Jie. "Alternative polyadenylation regulates the expression of the light harvesting gene LHCB4.1 in Arabidopsis mutant oxt6." Master's thesis, Miami University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=miami1322534400

    Chicago Manual of Style (17th edition)

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© 2011, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.