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Elucidation of Thermodynamic Parameters for a Host Cell Protein Acting on a HIV-1 Splicing Regulatory Element

Dewan, Nitika

Abstract Details

2011, Master of Science, Miami University, Chemistry and Biochemistry.
Alternative splicing is a key event in the HIV-1 replication cycle. Splice site A7 (ssA7) is a highly conserved site, playing a role in removing the Rev Responsive Element. The host protein, hnRNP A1 binds to splicing regulatory elements to inhibit ssA7 activity. To gain insight into the molecular basis of ssA7 antagonism, calorimetric titrations were performed using a C-terminal deletion mutant of hnRNP A1 (UP1) and a series of ssA7 single and double nucleotide mutations. Specifically, the thermodynamic profile (ΔG°, ΔH°, TΔS°, and Ka) were measured for each mutant under near physiological conditions, providing new insight into the physiochemical determinants of hnRNP A1-ssA7 molecular recognition.
Blanton Tolbert, PhD (Advisor)
Gary Lorigan, PhD (Committee Chair)
Carole -Dabney Smith, PhD (Committee Member)
David Tierney, PhD (Committee Member)
103 p.

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Citations

  • Dewan, N. (2011). Elucidation of Thermodynamic Parameters for a Host Cell Protein Acting on a HIV-1 Splicing Regulatory Element [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1323022700

    APA Style (7th edition)

  • Dewan, Nitika. Elucidation of Thermodynamic Parameters for a Host Cell Protein Acting on a HIV-1 Splicing Regulatory Element. 2011. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1323022700.

    MLA Style (8th edition)

  • Dewan, Nitika. "Elucidation of Thermodynamic Parameters for a Host Cell Protein Acting on a HIV-1 Splicing Regulatory Element." Master's thesis, Miami University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=miami1323022700

    Chicago Manual of Style (17th edition)