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Kaylee Gibson M.S. Thesis.pdf (2.36 MB)

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STRUCTURAL AND TOPOLOGICAL CHARACTERIZATION OF KCNE1 ELUCIDATED BY ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPYKCNE1

Gibson, Kaylee Roy
http://rave.ohiolink.edu/etdc/view?acc_num=miami1368099139

Abstract Details

2013, Master of Science, Miami University, Chemistry and Biochemistry.
KCNE1 (minK), a membrane protein known to modulate the function of the KCNQ1 voltage-gated potassium ion channel in the human heart, was successfully overexpressed, purified into detergent micelles, labeled, and incorporated into POPC:POPG lipid bilayers. CW-EPR and CW-EPR Power Saturation spectroscopy has been used to examine the structural properties of KCNE1 in lipid bilayer vesicles, as opposed to detergent micelles. Preliminary data was also collected for the development of an ESEEM EPR technique which will allow for quick determination of the local secondary structure of membrane proteins in lipid bilayers as opposed to micelles to better model the native membrane environment.
Gary Lorigan (Advisor)
Carole Dabney-Smith (Committee Chair)
75 p.
Biochemistry; Biophysics; Chemistry
EPR; KCNE1; ESEEM; Power Saturation; Biochemistry; Membrane Proteins; Lipid Vesicles

Recommended Citations

Citations

  • Gibson, K. R. (2013). STRUCTURAL AND TOPOLOGICAL CHARACTERIZATION OF KCNE1 ELUCIDATED BY ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPYKCNE1 [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1368099139

    APA Style (7th edition)

  • Gibson, Kaylee. STRUCTURAL AND TOPOLOGICAL CHARACTERIZATION OF KCNE1 ELUCIDATED BY ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPYKCNE1 . 2013. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1368099139.

    MLA Style (8th edition)

  • Gibson, Kaylee. "STRUCTURAL AND TOPOLOGICAL CHARACTERIZATION OF KCNE1 ELUCIDATED BY ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPYKCNE1 ." Master's thesis, Miami University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=miami1368099139

    Chicago Manual of Style (17th edition)

miami1368099139
411
© 2013, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.