Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


Master's thesis (Final).pdf (1.84 MB)

ETD Abstract Container

Abstract Header

Retrograde influences of peripheral nerve injury on uninjured neurons

Hawk, Kiel W.
http://rave.ohiolink.edu/etdc/view?acc_num=miami1387231538

Abstract Details

2013, Master of Science, Miami University, Cell, Molecular and Structural Biology (CMSB).
Changes in uninjured preganglionic neurons were assessed following axotomy of peripheral postganglionic axons arising from superior cervical ganglion (SCG) neurons at short-term (1 day and 7 days) and long-term (8 weeks and 12 weeks) survival time points. Immunohistochemistry was used to analyze choline acetyltransferase (ChAT) and synaptophysin expression in preganglionic terminals in the SCG, and western blotting was used to analyze BDNF and TrkB in the SCG and spinal cord. In the SCG a reduction in ChAT and synaptophysin immunoreactivity was observed at 7 days, and this reduction persisted at 8 and 12 weeks after injury. Changes in BDNF and TrkB were observed at 7 days post-injury in both the SCG and spinal cord. These findings characterized the effects of peripheral nerve injury on uninjured neurons that arise from the central nervous system and provided evidence that these effects may be regulated by retrograde neurotrophin signaling.
Lori Isaacson, Ph.D. (Advisor)
66 p.
Cellular Biology; Neurobiology; Neurosciences
BDNF; neurotrophin; neuotrophin receptor; TrkB; plasticity; retrograde; nerve injury; SCG; axotomy; NGF; synaptophysin; ChAT; immunohistochemistry; western; sympathetic

Recommended Citations

Citations

  • Hawk, K. W. (2013). Retrograde influences of peripheral nerve injury on uninjured neurons [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1387231538

    APA Style (7th edition)

  • Hawk, Kiel. Retrograde influences of peripheral nerve injury on uninjured neurons. 2013. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1387231538.

    MLA Style (8th edition)

  • Hawk, Kiel. "Retrograde influences of peripheral nerve injury on uninjured neurons." Master's thesis, Miami University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=miami1387231538

    Chicago Manual of Style (17th edition)

miami1387231538
260
© 2013, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.