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The spectral phasor approach as a tool for monitoring the autofluorescence of mitochondrial metabolism and its application to high pressure studies

Maltas, Jeffrey A

Abstract Details

2014, Master of Science, Miami University, Physics.
Many common causes of death, like cancer and Alzheimers, have a component involving metabolic dysfunction - thus the development of tools capable of extracting functional information is useful. NADH is a coenzyme prevalent in metabolic pathways related to mitochondrial function. Because NADH fluorescence depends on its conformational state, i.e., on its protein-bound state, it is a convenient non-invasive functional probe. Here we implement a spectral-phasor approach capable for measuring small (1 - 10 nm) shifts in NADH autofluorescence when suspensions of Saccharomyces cereviseae are perturbed using mitochondrial functional modi ers (cyanide and FCCP) and metabolic substrates (glucose and ethanol). The ability of a spectral-phasor approach in detecting small changes in autofluorescence is compared to the well-known spectral decomposition technique. Additionally, we explore the use of pressure in the 100-500 atm range as a metabolic perturbant by demonstrating the ability to monitor metabolic responses in a recently-constructed, high-pressure microperfusion system.
Paul Urayama, PhD (Advisor)
Samir Bali, PhD (Committee Member)
Perry Rice, PhD (Committee Member)
53 p.

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Citations

  • Maltas, J. A. (2014). The spectral phasor approach as a tool for monitoring the autofluorescence of mitochondrial metabolism and its application to high pressure studies [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1408122306

    APA Style (7th edition)

  • Maltas, Jeffrey. The spectral phasor approach as a tool for monitoring the autofluorescence of mitochondrial metabolism and its application to high pressure studies. 2014. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1408122306.

    MLA Style (8th edition)

  • Maltas, Jeffrey. "The spectral phasor approach as a tool for monitoring the autofluorescence of mitochondrial metabolism and its application to high pressure studies." Master's thesis, Miami University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=miami1408122306

    Chicago Manual of Style (17th edition)