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EGF signaling regulates adult muscle patterning in Drosophila

Vishal, Kumar
http://rave.ohiolink.edu/etdc/view?acc_num=miami1416505009

Abstract Details

2014, Doctor of Philosophy, Miami University, Biology.
During the Drosophila life cycle, two distinct sets of muscles develop: embryonic and adult. The embryonic set develops during the first day of the life cycle and establishes the muscles that will function during the 4 day larval period, mainly to facilitate crawling. The adult muscles execute behaviors such as flight and eclosion, and their development, unlike development in the embryo, is diverse and is influenced by neurons. Studies described in this thesis have tested the model that motor neurons signal to muscle organizer cells, which in turn communicate with myoblasts to influence proliferation. The overall goal of this thesis is to understand: i) How the interactions between motor neurons, organizer cells and myoblasts impact the size of the myoblast pool; ii) The role of myoblast proliferation in the regulation of fiber number; iii) The hierarchy of interaction between motor neurons, organizer cells and myoblasts; and iv) How EGF signaling is utilized in these interactions. Genetic manipulations were used to target transgenes to each of the three cell types, to disrupt the production and secretion of ligands and the transduction of signals. The effects of these manipulations were observed in adult muscle profiles, flight behavior and developmental events during metamorphosis. Collectively these studies have demonstrated that motor neurons are the primary signaling cells and act through organizer cells to regulate myoblast proliferation. Organizer cells act as both signaling and receiving cells. On the other hand, myoblasts are downstream of both motor neurons and organizer cells and act as secondary receiving cells. Motor neurons secrete the EGF ligands Spitz and Vein, which act through EGF receptors in organizer cells and myoblasts to scale up the myoblast pool. The pool size in turn dictates the muscle size and number, which impacts flight ability.
Joyce Fernandes (Advisor)
139 p.
Biology, Zoology
Drosophila, muscle, patterning, EGF signaling

Recommended Citations

Citations

  • Vishal, K. (2014). EGF signaling regulates adult muscle patterning in Drosophila [Doctoral dissertation, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1416505009

    APA Style (7th edition)

  • Vishal, Kumar. EGF signaling regulates adult muscle patterning in Drosophila . 2014. Miami University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1416505009.

    MLA Style (8th edition)

  • Vishal, Kumar. "EGF signaling regulates adult muscle patterning in Drosophila ." Doctoral dissertation, Miami University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=miami1416505009

    Chicago Manual of Style (17th edition)

miami1416505009
106
© 2014, some rights reserved.Creative Commons License EGF signaling regulates adult muscle patterning in Drosophila by Kumar Vishal is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Miami University and OhioLINK.