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Yaqi Wang thesis.pdf (3.08 MB)

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A study of the dADAR mRNA short isoform and a novel non-spliceosomal rnp-4f intron class in Drosophila

Wang, Yaqi
http://rave.ohiolink.edu/etdc/view?acc_num=miami1416934699

Abstract Details

2014, Master of Science, Miami University, Biology.
Adenosine Deaminase Acting on RNA (ADAR) is an RNA editing enzyme encoded by the gene ADAR. Drosophila ADAR (dADAR) pre-mRNA can produce full-length and truncated mRNA isoforms which terminate in intron 6 lacking the enzymatic domain. Very little information is available on truncated dADAR in Drosophila. In this research, DIG in situ localization results showed that both 0-2 h embryo dADAR mRNA isoforms are maternal and both isoforms are present in the developing central nervous system. Western blotting shows that both isoforms are translated into proteins in D. melanogaster and potential functions of the short isoform are discussed. In addition, by an extensive screen of polyadenylated EST clones from a cDNA library of D. melanogaster developmental stages, we have unexpectedly found rnp-4f transcripts containing an identically spliced 58-nt segment from some 0-4 h embryo clones. Excision of this novel intron, which is also present in related species, causes a frame-shift which completely changes the encoded C-terminus and results in a long unique 83 amino acid tract. Several interesting correlations exist between the Drosophila xbp1 gene intron and that of the novel rnp-4f intron, which belongs to a previously unknown intron class.
Jack Vaughn, PhD (Advisor)
Kathleen Killian, PhD (Committee Member)
Gary Janssen, PhD (Committee Member)
50 p.
Biology; Molecular Biology
dADAR gene; rnp-4f gene; Drosophila; intron

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Citations

  • Wang, Y. (2014). A study of the dADAR mRNA short isoform and a novel non-spliceosomal rnp-4f intron class in Drosophila [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1416934699

    APA Style (7th edition)

  • Wang, Yaqi. A study of the dADAR mRNA short isoform and a novel non-spliceosomal rnp-4f intron class in Drosophila . 2014. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1416934699.

    MLA Style (8th edition)

  • Wang, Yaqi. "A study of the dADAR mRNA short isoform and a novel non-spliceosomal rnp-4f intron class in Drosophila ." Master's thesis, Miami University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=miami1416934699

    Chicago Manual of Style (17th edition)

miami1416934699
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© 2014, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.