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Fluorescent derivatization of glycine using o-phthalaldehyde and captopril for the indirect determination of nitrite

Zhang, Ying
http://rave.ohiolink.edu/etdc/view?acc_num=miami1438036174

Abstract Details

2015, Master of Science, Miami University, Chemistry and Biochemistry.
In this research, we developed an indirect fluorescence method for determination of nitrite based on a two reaction sequence. The nitrosocaptopril produced by the first reaction between nitrite and excess captopril is not fluorescent. The fluorescent product produced by the residual captopril with glycine and o-phthalaldehyde (OPA) is then measured and related to the nitrite concentration. During the development of the method, we optimized comprehensively the reaction condition parameters, including the thiol regent for the two reactions, reaction temperature and pH value for the first reaction, and the choice of amino acid, pH value, the ratio of reactants, buffer concentration, reaction temperature, and time for the second fluorescence reaction. Under the optimized conditions, we investigated the effect of eight cations and ten anions on the fluorescence value. The results indicated there was good selectivity except for iodate and periodate, which could be removed by addition of a reducing agent. For the analytical figures of merit, the limit of detection and quantitation of nitrite were 0.8 nM and 2.5 nM, respectively. The fluorescence intensity was found to linearly decrease with increased nitrite from 0 nM to 140 nM. The method has shown to have good inter-day and intra-day precision.
Neil Danielson (Advisor)
Andre Sommer (Committee Chair)
Jonathan Scaffidi (Committee Member)
Scott Hartley (Committee Member)
53 p.
Analytical Chemistry
Nitrite detection; Captopril; Fluorescence

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Citations

  • Zhang, Y. (2015). Fluorescent derivatization of glycine using o-phthalaldehyde and captopril for the indirect determination of nitrite [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1438036174

    APA Style (7th edition)

  • Zhang, Ying. Fluorescent derivatization of glycine using o-phthalaldehyde and captopril for the indirect determination of nitrite. 2015. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1438036174.

    MLA Style (8th edition)

  • Zhang, Ying. "Fluorescent derivatization of glycine using o-phthalaldehyde and captopril for the indirect determination of nitrite." Master's thesis, Miami University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=miami1438036174

    Chicago Manual of Style (17th edition)

miami1438036174
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© 2015, some rights reserved.Creative Commons License Fluorescent derivatization of glycine using o-phthalaldehyde and captopril for the indirect determination of nitrite by Ying Zhang is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Miami University and OhioLINK.