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Synthesis of random and site-specific protein-polymer conjugates by RAFT polymerization

Falatach, Rebecca M
http://rave.ohiolink.edu/etdc/view?acc_num=miami1448299857

Abstract Details

2015, Master of Science, Miami University, Chemical, Paper and Biomedical Engineering.
Proteins and enzymes have long been used in a wide variety of applications from laundry detergents to therapeutics; however, instability under conditions such as high temperature, extreme pH, organic solvents, and the presence of proteases limit new applications.Combining natural proteins with synthetic polymers to create protein-polymer conjugates offers the opportunity to create new functional materials with improved activity and stability. In this study, random and site-specifically modified protein-polymer conjugates were synthesized by RAFT polymerization using grafting-to and grafting-from approaches. Polymer conjugates of the model proteins, lysozyme and chymotrypsin, were synthesized by random modification and the enzyme activity, stability, and number of attachments were determined. Site-specific modification of green fluorescent protein was achieved using the LAP/Lipoic Acid Ligase system and RAFT polymerization. These bioconjugates were characterized to determine the number of CTA attachments and polymer length.
Jason Berberich (Advisor)
Richard Page (Committee Member)
Dominik Konkolewicz (Committee Member)
Jessica Sparks (Committee Member)
87 p.
Chemical Engineering
RAFT polymerization, enzymes, catalytic activity, site-specific

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Citations

  • Falatach, R. M. (2015). Synthesis of random and site-specific protein-polymer conjugates by RAFT polymerization [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1448299857

    APA Style (7th edition)

  • Falatach, Rebecca. Synthesis of random and site-specific protein-polymer conjugates by RAFT polymerization. 2015. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1448299857.

    MLA Style (8th edition)

  • Falatach, Rebecca. "Synthesis of random and site-specific protein-polymer conjugates by RAFT polymerization." Master's thesis, Miami University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=miami1448299857

    Chicago Manual of Style (17th edition)

miami1448299857
394
© 2015, some rights reserved.Creative Commons License Synthesis of random and site-specific protein-polymer conjugates by RAFT polymerization by Rebecca M Falatach is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Miami University and OhioLINK.