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Structural Studies of KCNE1 in Lipid Bilayers with Magnetic Resonance Spectroscopy and Characterization of Membrane Mimetic Lipodisq Nanoparticles

Zhang, Rongfu
http://rave.ohiolink.edu/etdc/view?acc_num=miami1464777769

Abstract Details

2016, Doctor of Philosophy, Miami University, Cell, Molecular and Structural Biology (CMSB).
In general, structural studies of membrane proteins are more difficult than soluble proteins mainly due to their intrinsic hydrophobic properties. The lack of suitable biophysical techniques and proper membrane mimetics has greatly hindered the research of membrane proteins. In this dissertation, efforts have been made on the development of new biophysical techniques and membrane mimetics using the model membrane protein KCNE1. Chapter 2 focuses on utilizing 31P solid-state NMR spectroscopy to examine the changes in dynamic properties of synthetic lipid bilayers upon incorporation of the membrane protein KCNE1. Chapters 3 and 4 aim at the characterization of a newly-developed membrane mimetic termed lipodisq nanoparticles by dynamic light scattering (DLS), solid-state NMR spectroscopy, and transmission electron microscopy (TEM) techniques. This alternative membrane mimetic broadens the choices of the synthetic lipid systems for structural studies of membrane proteins. Chapters 5 and 6 successfully demonstrate the application of electron spin echo envelope modulation (ESEEM) spectroscopy coupled with site-directed spin-labeling (SDSL) for the local secondary structure determination of the membrane protein KCNE1 in both micelle and lipid bilayer environments. This advanced pulsed EPR technique provides a fast and straightforward method which can be adapted to the study of membrane proteins with unknown structures in a native-like environment.
Gary Lorigan (Advisor)
119 p.
Biochemistry
KCNE1, solid-state NMR spectroscopy, ESEEM, Lipodisq nanoparticles, MTSL

Recommended Citations

Citations

  • Zhang, R. (2016). Structural Studies of KCNE1 in Lipid Bilayers with Magnetic Resonance Spectroscopy and Characterization of Membrane Mimetic Lipodisq Nanoparticles [Doctoral dissertation, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1464777769

    APA Style (7th edition)

  • Zhang, Rongfu. Structural Studies of KCNE1 in Lipid Bilayers with Magnetic Resonance Spectroscopy and Characterization of Membrane Mimetic Lipodisq Nanoparticles. 2016. Miami University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1464777769.

    MLA Style (8th edition)

  • Zhang, Rongfu. "Structural Studies of KCNE1 in Lipid Bilayers with Magnetic Resonance Spectroscopy and Characterization of Membrane Mimetic Lipodisq Nanoparticles." Doctoral dissertation, Miami University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=miami1464777769

    Chicago Manual of Style (17th edition)

miami1464777769
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© 2016, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.