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GENOME WIDE STUDIES OF THE ROLE OF POLY(A) TAIL LENGTH AND POLY(A) FACTORS IN PLANTS

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2016, Doctor of Philosophy, Miami University, Biology.
Chapter One is an introduction of the whole dissertation. I described the background information about current studies on poly(A) factors and poly(A) tail length in animals, plants and yeast. In Chapter Two we used a Minigene containing RNAi-induced phytoene desaturase (PDS) silencing structure, and a strong polyadenylation signal to select polyadenylation mutants. Four potential poly(A) factor mutants with different phenotypes were generated. In Chapter Three, we designed a novel protocol to accurately measure poly(A) tail lengths by taking advantage of a new DNA sequencing platform by Pacific Biosciences (PacBio) Inc. The authenticity of poly(A) tail length was further confirmed with Sanger sequencing by randomly picking three genes with varied poly(A) tail lengths for comparison. The results suggested that the protocol could accurately measure poly(A) tail length at a transcriptome scale. In Chapter Four we explored the role of poly(A) tail length during seed germination by using the novel protocol we designed. Based on poly(A) tail length and re-polyadenylation time, 10 stored transcripts with short poly(A) tails were identified as potential regulators of seed germination in Arabidopsis. They could be classified into 5 groups by re-polyadenylation times in germination phases. In Chapter Five, the functions of the poly(A) tail length in response to heat stress were studied. Experiments with both gradual and abrupt heat stress treatments showed that two different re-polyadenylation patterns existed among 14 transcripts. One of them was independent of heat treatment regime. The other re-polyadenylation pattern was limited to severity of heat shock. Each of them covers seven transcripts. In Chapter Six, I conclude the finding of my current studies and give perspectives for the research directions of poly(A) factors and poly(A) tail length.
Quinn li (Advisor)
121 p.

Recommended Citations

Citations

  • JIE, W. (2016). GENOME WIDE STUDIES OF THE ROLE OF POLY(A) TAIL LENGTH AND POLY(A) FACTORS IN PLANTS [Doctoral dissertation, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1480497559325595

    APA Style (7th edition)

  • JIE, WANG. GENOME WIDE STUDIES OF THE ROLE OF POLY(A) TAIL LENGTH AND POLY(A) FACTORS IN PLANTS . 2016. Miami University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1480497559325595.

    MLA Style (8th edition)

  • JIE, WANG. " GENOME WIDE STUDIES OF THE ROLE OF POLY(A) TAIL LENGTH AND POLY(A) FACTORS IN PLANTS ." Doctoral dissertation, Miami University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=miami1480497559325595

    Chicago Manual of Style (17th edition)