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Zhu_Yingdong_OhioLINK_12_6_16.pdf (1.85 MB)
ETD Abstract Container
Abstract Header
Analysis of Novel 5'-UTR Polyadenylation Sites in Arabidopsis thaliana
Author Info
Yingdong, Zhu
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=miami1480717686996059
Abstract Details
Year and Degree
2016, Master of Science, Miami University, Cell, Molecular and Structural Biology (CMSB).
Abstract
Messenger RNA (mRNA) polyadenylation is an indispensable step during post-transcriptional pre-mRNA processing in eukaryotes. The usage of one poly(A) site over another is known as alternative polyadenylation (APA). APA has been implicated in gene expression regulation through its role of selecting the ends of a transcript. Recent studies of polyadenylation profiles in the Arabidopsis database predicted that a portion of the poly(A) sites are located in the 5'-UTR. Here, we first describe 5'-UTR-APA and verify existence of independent polyadenylated 5'-UTR transcripts, arising due to alternative polyadenylation in the 5'-UTRs of the corresponding genes. We used 3'-RACE and sequencing to validate poly(A) sites and Northern blot to show that the observed short upstream transcripts do not arise from the 3'-end of a previously unrecognized convergent gene. We hypothesize that 5'-UTR polyadenylation may represent a gene silencing mechanism, and that truncated 5'-UTR transcripts may have functions, either existing as polyadenylated non-coding RNAs, or potentially to regulate gene expression by encoding a short peptide, which we call a 5'-UTR ORF. We propose a model to explain the features and functions of 5'-UTR ORF as well as the relationships between upstream ORF (uORF) and major ORF (mORF).
Committee
Jack Vaughn (Advisor)
Chun Liang (Committee Chair)
Yoshinori Tomoyasu (Committee Member)
Subject Headings
Biology
Keywords
5-UTR
;
alternative polyadenylation
;
open reading frame
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Citations
Yingdong, Z. (2016).
Analysis of Novel 5'-UTR Polyadenylation Sites in Arabidopsis thaliana
[Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1480717686996059
APA Style (7th edition)
Yingdong, Zhu.
Analysis of Novel 5'-UTR Polyadenylation Sites in Arabidopsis thaliana.
2016. Miami University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=miami1480717686996059.
MLA Style (8th edition)
Yingdong, Zhu. "Analysis of Novel 5'-UTR Polyadenylation Sites in Arabidopsis thaliana." Master's thesis, Miami University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=miami1480717686996059
Chicago Manual of Style (17th edition)
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Document number:
miami1480717686996059
Download Count:
191
Copyright Info
© 2016, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.