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Hailey Riggs' Masters Thesis 2017 COMPLETE.pdf (1.92 MB)
ETD Abstract Container
Abstract Header
Mycoplasma pneumoniae
protein P30: Stability, interactions, and function
Author Info
Riggs, Hailey Erin
ORCID® Identifier
http://orcid.org/0000-0002-7595-6547
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=miami1511952462541533
Abstract Details
Year and Degree
2017, Master of Science, Miami University, Microbiology.
Abstract
Mycoplasma pneumoniae
infections are a leading cause of community-acquired pneumonia. There has been a recent surge in the incidence of antibiotic-resistant
M. pneumoniae
infections. Consequently, developing new targets to fight infections has become a priority. Both cytadherence and gliding motility are required for
M. pneumoniae
to be pathogenic, but their underlying mechanisms are unclear. These processes are mediated through the attachment organelle, a membrane-bound projection of the cell. Transmembrane protein P30 localizes to the tip of the attachment organelle and is required for both processes. The C-terminal region of P30 contains a series of imperfect proline-rich repeats (PRRs). Mutant P30 lacking these PRRs is unstable. We hypothesize that the PRRs of the C-terminal region of P30 contribute to the stability and functions of P30 through facilitating interactions with itself and/or other proteins. Chemical cross-linking and immunoblot analysis were performed to determine the binding partners of P30. Site-directed mutagenesis was used to identify roles of specific proline residues in the stability, structure, and functions of P30. This work demonstrated that P30 is present in putative homomeric and heteromeric complexes, and there is a complicated relationship between the proline residues and their roles in the structure and function of P30.
Committee
Mitchell Balish, PhD (Advisor)
Joseph Carlin, PhD (Committee Member)
Natosha Finley, PhD (Committee Member)
Pages
72 p.
Subject Headings
Microbiology
Keywords
Mycoplasma
;
protein
;
proline
;
gliding motility
;
stability
;
cytadherence
Recommended Citations
Refworks
EndNote
RIS
Mendeley
Citations
Riggs, H. E. (2017).
Mycoplasma pneumoniae
protein P30: Stability, interactions, and function
[Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1511952462541533
APA Style (7th edition)
Riggs, Hailey.
Mycoplasma pneumoniae
protein P30: Stability, interactions, and function.
2017. Miami University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=miami1511952462541533.
MLA Style (8th edition)
Riggs, Hailey. "
Mycoplasma pneumoniae
protein P30: Stability, interactions, and function." Master's thesis, Miami University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=miami1511952462541533
Chicago Manual of Style (17th edition)
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Document number:
miami1511952462541533
Download Count:
279
Copyright Info
© 2017, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.