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Mycoplasma pneumoniae protein P30: Stability, interactions, and function

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2017, Master of Science, Miami University, Microbiology.
Mycoplasma pneumoniae infections are a leading cause of community-acquired pneumonia. There has been a recent surge in the incidence of antibiotic-resistant M. pneumoniae infections. Consequently, developing new targets to fight infections has become a priority. Both cytadherence and gliding motility are required for M. pneumoniae to be pathogenic, but their underlying mechanisms are unclear. These processes are mediated through the attachment organelle, a membrane-bound projection of the cell. Transmembrane protein P30 localizes to the tip of the attachment organelle and is required for both processes. The C-terminal region of P30 contains a series of imperfect proline-rich repeats (PRRs). Mutant P30 lacking these PRRs is unstable. We hypothesize that the PRRs of the C-terminal region of P30 contribute to the stability and functions of P30 through facilitating interactions with itself and/or other proteins. Chemical cross-linking and immunoblot analysis were performed to determine the binding partners of P30. Site-directed mutagenesis was used to identify roles of specific proline residues in the stability, structure, and functions of P30. This work demonstrated that P30 is present in putative homomeric and heteromeric complexes, and there is a complicated relationship between the proline residues and their roles in the structure and function of P30.
Mitchell Balish, PhD (Advisor)
Joseph Carlin, PhD (Committee Member)
Natosha Finley, PhD (Committee Member)
72 p.

Recommended Citations

Citations

  • Riggs, H. E. (2017). Mycoplasma pneumoniae protein P30: Stability, interactions, and function [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1511952462541533

    APA Style (7th edition)

  • Riggs, Hailey. Mycoplasma pneumoniae protein P30: Stability, interactions, and function. 2017. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1511952462541533.

    MLA Style (8th edition)

  • Riggs, Hailey. "Mycoplasma pneumoniae protein P30: Stability, interactions, and function." Master's thesis, Miami University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=miami1511952462541533

    Chicago Manual of Style (17th edition)