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Full master thesis_Fanyi Zhong_Miami U-04262018.pdf (7.56 MB)
ETD Abstract Container
Abstract Header
DEVELOPMENT AND APPLICATIONS OF HPLC-MS/MS BASED METABOLOMICS
Author Info
Zhong, Fanyi
ORCID® Identifier
http://orcid.org/0000-0001-9073-1437
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=miami1524792637748877
Abstract Details
Year and Degree
2018, Master of Science, Miami University, Chemistry and Biochemistry.
Abstract
High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) is a powerful and mainstream analytical platform widely used in both targeted and untargeted metabolomics analysis, thanks to its high sensitivity, selectivity, and reproducibility. Therefore, it was chosen as the major technique of our research and was used throughout my thesis projects. In this thesis work, the development of metabolomics techniques from targeted analysis to hybrid metabolomics approach, has been introduced. This thesis starts with an introduction focused on the general workflow of hybrid metabolomics and the analytical considerations of the optimized hybrid approach. This review is then followed by chapter 2 and 3, two published studies that demonstrated the utility of targeted metabolomics methods to different biomedical applications. Specifically, chapter 2 compared the impact of ultrafine particles from petrodiesel and biodiesel combustion to gut bacterial metabolism; chapter 3 applied targeted metabolomics to differentiate metabolic syndrome from obese patients who did not qualify as be metabolic syndrome via their metabolic profiles. In chapter 4, a hybrid metabolomics workflow was developed to integrate time staggered and mass staggered ion lists with globally optimized targeted-mass spectrometry (ts/ms-GOT-MS); a broader metabolome coverage with an improved analytical performance was achieved.
Committee
Jiangjiang Zhu (Advisor)
Neil Danielson (Committee Chair)
David Tierney (Committee Member)
Richard Page (Committee Member)
Pages
177 p.
Subject Headings
Chemistry
Keywords
metabolomics
;
targeted metabolomics
;
untargeted metabolomics
;
hybrid metabolomics
;
HPLC-MS
;
method development
;
applications
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Refworks
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Citations
Zhong, F. (2018).
DEVELOPMENT AND APPLICATIONS OF HPLC-MS/MS BASED METABOLOMICS
[Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1524792637748877
APA Style (7th edition)
Zhong, Fanyi.
DEVELOPMENT AND APPLICATIONS OF HPLC-MS/MS BASED METABOLOMICS.
2018. Miami University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=miami1524792637748877.
MLA Style (8th edition)
Zhong, Fanyi. "DEVELOPMENT AND APPLICATIONS OF HPLC-MS/MS BASED METABOLOMICS." Master's thesis, Miami University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=miami1524792637748877
Chicago Manual of Style (17th edition)
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Document number:
miami1524792637748877
Download Count:
621
Copyright Info
© 2018, some rights reserved.
DEVELOPMENT AND APPLICATIONS OF HPLC-MS/MS BASED METABOLOMICS by Fanyi Zhong is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Miami University and OhioLINK.