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MASTER THESIS__HAORONG.pdf (6.31 MB)

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Development of Secondary Electrospray Ionization Tandem Mass Spectrometry and its Application in Bacterial Headspace Volatile Organic Compounds Detection

Li, Haorong
http://rave.ohiolink.edu/etdc/view?acc_num=miami1533210755679687

Abstract Details

2018, Master of Science, Miami University, Chemistry and Biochemistry.
In this thesis work, we developed a secondary electrospray ionization tandem mass spectrometry (SESI-MS/MS) instrument with a homemade SESI-MS front end, for sensitive, reproducible, and selective detection of headspace volatile organic compounds (VOCs) from in vitro bacterial culture. We applied the SESI-MS/MS to examine the VOC metabolites of a pair of isogenic methicillin-susceptible and resistant Staphylococcus aureus (MSSA and MRSA) strains. Twelve organic acids and eight amino acids were specifically targeted. Our results showed that MSSA and MRSA could be clearly differentiated based on their headspace metabolic profiles. Dramatic changes in the VOC metabolic profiles of MSSA and MRSA were also captured after antibiotic perturbations. Furthermore, we incorporated SESI-MS/MS with a hybrid metabolomics technique, globally optimized targeted mass spectrometry (GOT-MS), and developed the GOT SESI-MS/MS method. Both targeted and GOT SESI-MS/MS methods were applied to detect volatile metabolites from the headspace of in vitro gut microbial cultures in a human colonic model (HCM). Our results showed that the targeted and GOT SESI-MS/MS methods showed comparable analytical performance and both methods could clearly differentiate the gut microbial samples from different stages of HCM. Both targeted and GOT SESI-MS/MS could be useful tools for monitoring gut microbial metabolism and their response to perturbations.
Jiangjiang Zhu (Advisor)
Neil Danielson (Committee Chair)
Carole Dabney-Smith (Committee Member)
David Tierney (Committee Member)
126 p.
Analytical Chemistry
SESI-MSMS; MRSA; antibiotics; GOT-MS

Recommended Citations

Citations

  • Li, H. (2018). Development of Secondary Electrospray Ionization Tandem Mass Spectrometry and its Application in Bacterial Headspace Volatile Organic Compounds Detection [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1533210755679687

    APA Style (7th edition)

  • Li, Haorong. Development of Secondary Electrospray Ionization Tandem Mass Spectrometry and its Application in Bacterial Headspace Volatile Organic Compounds Detection. 2018. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1533210755679687.

    MLA Style (8th edition)

  • Li, Haorong. "Development of Secondary Electrospray Ionization Tandem Mass Spectrometry and its Application in Bacterial Headspace Volatile Organic Compounds Detection." Master's thesis, Miami University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=miami1533210755679687

    Chicago Manual of Style (17th edition)

miami1533210755679687
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© 2018, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.