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NMuhammad CMSB MS Thesis.pdf (1.25 MB)

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INVESTIGATING THE PORE COMPOSITION OF THE CHLOROPLAST TWIN ARGININE TRANSPORT SYSTEM

Muhammad, Nefertiti
http://orcid.org/0000-0001-7904-6498
http://rave.ohiolink.edu/etdc/view?acc_num=miami1543609222190572

Abstract Details

2018, Master of Science, Miami University, Cell, Molecular and Structural Biology (CMSB).
The cpTat system transports fully folded proteins into the thylakoid using the proton motive force and, together, cpTatC, Tha4, and Hcf106 transport precursors across the bilayer membrane. Tha4 is predicted to form the translocation pore; however, Hcf106 may also have a role because of the large size of some cpTat substrates and its homology to Tha4. In this study, direct binding between Hcf106 and a cpTat substrate, the Oxygen Evolving Complex precursor (pOE17), was investigated. Prior work showed direct binding to Tha4 for four variants of the substrate (pOE17-D68C, -S84C, -K99C, and -T115C) using cysteine crosslinking. The current study showed little to no direct binding between Hcf106 and the last variant of the substrate (pOE17-T115C); although, that variant could interact with Tha4. Together with precursor-Tha4 interaction maps, we concluded that both cpTat components bind directly to the substrate and the strongest interactions occur at the C-tail and the C-terminal end of the amphipathic helix (APH) region. This study also investigates whether cpTat substrates stay in contact with Hcf106 throughout transport. Protease treatments were performed on crosslinked Hcf106 and pOE17 at the C-tail and N-terminal residues of Hcf106. Unexpectedly, membrane-protected and -unprotected crosslinked products were detected in the N-terminus of Hcf106. The paper presents limitations with the study and directions for future research.
Carole Dabney-Smith (Advisor)
Gary Lorigan (Committee Member)
Anne Hagerman (Committee Member)
56 p.
Biochemistry; Biology
Hcf106, Chloroplast Twin Arginine Translocation System

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Citations

  • Muhammad, N. (2018). INVESTIGATING THE PORE COMPOSITION OF THE CHLOROPLAST TWIN ARGININE TRANSPORT SYSTEM [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1543609222190572

    APA Style (7th edition)

  • Muhammad, Nefertiti. INVESTIGATING THE PORE COMPOSITION OF THE CHLOROPLAST TWIN ARGININE TRANSPORT SYSTEM. 2018. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1543609222190572.

    MLA Style (8th edition)

  • Muhammad, Nefertiti. "INVESTIGATING THE PORE COMPOSITION OF THE CHLOROPLAST TWIN ARGININE TRANSPORT SYSTEM." Master's thesis, Miami University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=miami1543609222190572

    Chicago Manual of Style (17th edition)

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This open access ETD is published by Miami University and OhioLINK.